Journal of Environmental Biology

Micropropagation of Miniature Rose cv. Ice Fairy: Impact of growth regulators on in-vitro shooting and rooting

G. Kumari¹, M. Sood¹ and P. Kumari^1,2*     

¹Agrotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur-176 061, India

²Academy of Scientific and Innovative Research (AcSIR), Ghaziabad-201 002, India

Received: 17 January 2025                   Revised: 26 March 2025                   Accepted: 28 May 2025

*Corresponding Author Email : poonamjaswalfls@gmail.com                  *ORCiD: https://orcid.org/0000-0001-7066-3000

Abstract

Aim: The aim of this experiment was to optimize a procedure for in-vitro micro-propagation of Miniature Rose cv. Ice Fairy (Rosa hybrida L.) utilizing the nodal segments as an explant.

Methodology: The impact of cytokinins and gibberellins was investigated under in-vitro conditions. Different levels of BAP (6- Benzylaminopurine) (1, 1.5, 2, 2.5 mg l^-1), focusing on the developmental stages of explant whereas GA₃ (Gibberellic acid) was employed for shoot elongation and flowering. Activated charcoal (1 g l^-1) was added into the medium to inhibit phenolic oxidation.

Results: The highest shoot initiation was found in treatment T₄ (94 %) with culture survival rate of 86 % when Murashige and Skoog (MS) medium was supplemented with 2.5 mg l^-1 BAP and 1 g l^-1 activated charcoal. The highest number of elongated shoots were noted in the medium containing 2.5 mg l^-1 BAP and 1 mg l^-1 GA₃. It was observed that MS media supplemented with BAP was successful for shooting as well as rooting. In-vitro plantlets with 5 to 6 roots, each measuring 4-5 cm in length were carefully removed and transplanted into sterilized glass jars. The jars had a mixture of sand, coco-peat and soil in 1:1:2 (v/v) ratio, which was moistened with 30 % of MS basal liquid medium.

Interpretation: These findings may therefore contribute towards the commercial micropropagation of miniature roses.

Key words: 6-Benzylaminopurine, In-vitro propagation, Miniature rose, MS media, Phenolic oxidation

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