Journal of Environmental Biology

Comparison study on low-density polyethylene film biodegradation potential of Achoria grisella and Galleria mellonella larvae

N. Kapahi, L. Marwaha*, M. Kaur and G. Bawa     

Department of Zoology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara-144 411, India

*Corresponding Author Email : lovleenmarwaha@gmail.com                  *ORCiD: https://orcid.org/0000-0003-4250-5317

Received: 14.03.2023                                                                                        Revised: 24.05.2023                                                                          Accepted: 07.06.2023

Abstract

Aim: The current research investigates biodegradation of Low-Density Polyethylene (LDPE) facilitated with and without gut microbiota in Galleria mellonella and Achoria grisella.

Methodology: A batch of 50 larvae of Galleria mellonella and Achoria grisella administered with antibiotics (A group) along with larvae not fed with antibiotics (WA group) were exposed to LDPE film. After biodegradation, the weight loss of degraded LDPE film, Scanning Electron Microscopy, Fourier Transform Infrared Spectroscopy, Thermogravimetric Analysis, and Gas Chromatography-Mass Spectroscopy analysis were performed to confirm the plastic biodegradation potential of selected insects.

Results: In a and WA groups, the post-biodegradation for a selected species of wax moth exhibited weight loss of 2.763%, 0.789%, 5.403% and 1.481%, respectively. The SEM images revealed that both A and WA groups were capable of LDPE film disintegration. The FTIR of biodegraded film confirmed the deterioration of LDPE by indicating a change in the typical peaks of C−H and H−C−H bonds that points out the biodegradation of polyethylene.Further, the thermogravimetric analysis showed a substantial mass loss in the LDPE flakes consumed by the insects, demonstrating a decline in the thermal stabilities of disintegrated polymers. GC-MS of frass validated the presence of many component groups, including alkanes, alcohols, esters, ethers and acids, which confirmed the biodegradation of LDPE.

Interpretation: Wax worms possess the capacity to biodegrade LDPE, independent of gut microbiota

Key words: Achoria grisella, Antibiotic, Galleria mellonella, Low-density polyethylene

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