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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP
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    Abstract - Issue Jul 2021, 42 (4)                                     Back


nstantaneous and historical temperature effects on a-pinene

Biomolecular characterization and efficacy evaluation of pathogen Erwinia tracheiphila against plant oils

 

S. Pattanayak1, G. Biswal2 and S. Das1* 

1Department of Plant Pathology, MS Swaminathan School of Agriculture, Centurion University of Technology and Management, Gajapati- 761 211, India

2Department of Plant Pathology, College of Agriculture, Orissa University of Agriculture and Technology, Bhubaneswar-751 003, India

*Corresponding Author Email : siddhartha.das10@gmail.com

 

Received: 16.05.2020                                                                 Revised: 23.08.2020                                                   Accepted: 07.11.2020

 

 

Abstract

Aim: To investigate patho-dyanamics of Erwinia tracheiphila on Cucurbita pepo with biochemical, molecular characterization and eco-friendly management approaches.

Methodology: Variability study of Erwinia tracheiphila was done by collecting the isolates from different places in Odisha. Disease severity was calculated and analyzed from 2016-18 to predict trend of emergence and disease progress. Molecular detection of pathogen was done through 16S rRNA sequencing. Biochemical characterization and identification of pathogenic nature of Erwinia tracheiphila was performed through Ortho-Nitrophenyl β-Galactoside, Esculin hydrolysis, Citrate and Malonate utilization in the presence of different carbohydrate molecules. Efficacy of eight different plant oils were tested by in-vitro inhibition zone (IVIZ) assay against Erwinia tracheiphila as eco-friendly management approaches.

Results: Analysis of disease severity data from 2016-18 ranged between 29% to 33%. Molecular detection of causal pathogen was further confirmed by 16S rRNA region sequencing with 99% identity of NCBI-GenBank published Erwinia tracheiphila (MK356446 and MK356441) database and phylogenetic analysis. The results of IVIZT revealed that the clove oil treatment showed maximum inhibitory effect on the growth of Erwinia tracheiphila followed by mustard oil and neem oil. Sesamum oil showed the lowest efficacy against Erwinia tracheiphila. Optimum temperature range between 25-28°C, 80-85% relative humidity and 150-180 mm average rainfall triggered the disease progress and turned to be a severe one under experimental agro-climatic situation.      

Interpretation: Biochemical characterization and molecular detection of causal isolate confirmed the pathogen as Erwinia tracheiphila, causing wilt disease of Cucurbita pepo. This study also revealed the potential efficacy of clove oil and mustard oil against Erwinia tracheiphila as compared to other selected plant oils.

Key words: Erwinia tracheiphila, Molecular detection, Patho-dyanamics, Plant oils

 

 

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