Journal of Environmental Biology

Genotoxicity effects of medicinal plants extracts against bacterial species, Mycoplasma hominis

M.H. Yassin¹, Y. Alghamdi¹, E.H. Mohamed^1,2, S.A. Mostafa^1,3, A. Merghani⁴, H.H. Amer^5,6, S.H. Alotaibi⁵, M.M. Soliman^7,8, H. Nasr-eldeen⁹ and M.M. Hassan¹⁰* 

¹Department of Biology, Turabah University College, Taif University, 21995, Saudi Arabia

²Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44519, Egypt

³Immunobiology and Immunopharmacology Unit, Animal Reproduction Research Institute, Giza, 11753, Egypt

⁴Department of Molecular Genetics, Aljazeera University, Al-khartoum, 34228, Sudan.

⁵Chemistry Department, University College of Turabah, Taif University, Turabah, Taif, 21995, Saudi Arabia.

⁶Animal Medicine and Infectious Diseases Department, Faculty of Veterinary Medicine, University of Sadat City, Sadat, 14174,Egypt

⁷Clinical Laboratory Sciences Department, Turabah University College, Taif University, Taif, 21944, Saudi Arabia

⁸Biochemistry Departemt, Faculty of Veterinay Medicine, Benha University,  Toukh, 13736, Egypt

⁹Microbiology Laboratory, King Faisal Medical Complex, Taif, 11211, Saudi Arabia

¹⁰Department of Biology, College of Science, Taif University, Taif, 21944, Saudi Arabia

*Corresponding Author Email : m.khyate@tu.edu.sa

Received: 17.08.2020                                                                        Revised: 26.11.2020                                                             Accepted: 01.01.2021

Abstract

Aim: To assess the antimicrobial activity and genotoxicity of three medicinal plants used by Saudi Arabian people as traditional medicine against Mycoplasma hominis.

Methodology: Different concentrations of Syzygium aromaticum (clove), Vachellia nilotica (acacia), and Thyme vulgaris (thyme) extracts were used as antimicrobial agents against M. hominis, and their lethal effects on Mycoplasma genome DNA were analyzed using repetitive element PCR(Rep-PCR).

Results: The aqueous extracts of clove and Acacia at 3.125 mg ml^-1 were found to be active antimicrobials against three tested Mycoplasm. Thyme extract exhibited antimicrobial activity at 12.5 mg ml^-1. Moreover, this extract revealed potent lethal activities as growth turbidity decreased with increasing concentration or exposure time as compared to untreated Mycoplasma. The results of Rep-PCR clearly indicate that changes occured in the number of genetic bands in treated Mycoplasma at certain concentrations as compared to untreated Mycoplasma.               

Interpretation: These results indicate the possibility of using these extracts as a source of antibacterial compounds for treating infections caused by Mycoplasma.      

Key words: Antimicrobial activity, Genotoxicity, Mycoplasma hominis, Medicinal plants, S. aromaticum

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