Inbreeding effect
towards genetic markers and thermal tolerance in guppy Poecilia reticulate
F. Syukri1,2,
H.H. Nadiatul3, T. Nakajima1 and M. Nakajima1*
?
1Laboratory of
Population Genetic Informatics, Graduate School of Agriculture, Tohoku
University, Miyagi, 981-8555, Japan
2International
Institute of Aquaculture and Aquatic Sciences, Universiti Putra Malaysia,
Serdang, 43400, Selangor Darul Ehsan, Malaysia
3Department of
Biology, Faculty of Science, Universiti Putra Malaysia, Serdang, 43400,
Selangor Darul Ehsan, Malaysia
*Corresponding Author Email : masamichi.nakajima.b6@tohoku.ac.jp
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Abstract
Aim:
The guppy was used to demonstrate the effect of continues full-sib mating to
the genetic changes and occurrence of inbreeding depression through heat
stress tolerance.
Methodology: Inbreeding depression were assessed from data
collected from microsatellite markers and thermal tolerance experiment. The
AY and NA1 strains were selected for this experiment.
Results:
With the progress in the inbred generations, significant decrease in thermal
tolerance was observed. The observed (Ho) and expected (He) heterozygosity
also decreased, indicating the on going inbred process. Significant
correlation was observed only in the NA1 strain between thermal tolerances with
multilocus heterozygosity (MLH) experiment both in Internal Relatedness (IR)
and Standardize Heterozygosity (SH), respectively.
Interpretation: MLH can be used to determent inbreeding
depression only when the samples are severely inbred?
Keywords: Guppy, inbreeding, Microsatellite, Thermal tolerance
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