Journal of Environmental Biology

Application of pulsed field gel electrophoresis for molecular characterization of vancomycin resistant Staphylococcus aureus isolates from hospitals

A. Talwar¹* and S. Kumar² 

¹Department of Microbiology, Doon (PG) Paramedical College and Hospital, Dehradun-248 001, India

²Institute of Pharma Sciences, Chandigarh University, Chandigarh-160 036, India

*Corresponding Author Email : amitabhtalwar96@gmail.com

Paper received: 15.05.2019??????? ?????????????????????????????????????? Revised received: 19.09.2019? ?? ???????? ???????????????????????Accepted: 27.11.2019

Abstract

Aim: The present study was conducted to characterize VRSA isolates on the basis of pulsed field gel electrophoresis (PFGE) and the presence of spa gene, recovered from different Doon Valley Hospitals.

Methodology: Six VRSA isolates were analyzed using PFGE and spa typing. spa gene coded Protein A was used as a genetic marker for the characterization of Staphylococcus aureus isolates. Dendrogram were constructed on the basis of unweighted pair group method with arithmetic means (UPGMA method) for clusters analyses.

Results: Dendrogram finally showed two major banding patterns at about 85% similarity designated as PFGE type A and PFGE type B exhibiting differences of 4-6 bands. The length of spa gene varied from 1200 to 1500 bp, showing variation in length. The most prevalent length was 1200bp.

Interpretation: VRSA clones are closely related as PFGE results showed less diversity. Only three strains exhibited spa gene.??

Key words: Pulse field gel electrophoresis, Spa gene, Staphylococcus aureus, Vancomycin resistant, VRSA clones

Copyright ? 2020 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).