Journal of Environmental Biology

Genetic distance and phylogenetic studies of two Indian snakehead and one carp fish species

Paper received: 03.08.2018??????? ???????? Revised received: 11.02.2019??????? ???????????? Re-revised received: 25.04.2019??????????? ???????????????????????????? Accepted: 08.05.2019

Authors Info

S.K. Bhagabati¹*, C.S. Prakash²,

G. He³, M. Yuan³, Y. Zhao³,

K. Kalita⁴, I.A. Hussain⁵ and

R. Dutta¹

¹Department of Aquatic Environment

Management, College of Fisheries,

Assam Agricultural University,

Raha-782 103, India

²College of Arts and Sciences,

70-402 Kenney? Hall, Tuskegee University, Tuskegee, AL 36088, USA

³College of Agriculture, Environment

and Nutrition Sciences, Henderson Hall, Tuskegee University, Tuskegee, AL 36088, USA

⁴Department of Pathology, College of

Veterinary Sciences, Assam Agricultural University,

Khanapara-781 022, India

⁵Department of Fish Processing Technology, College of Fisheries,

Assam Agricultural University,?

Raha-782 103, India

*Corresponding Author Email :

sskbk2002@gmail.com

Abstract

Aim: To study genetic distance and phylogenetic relationship of two different stock of three important fish species, i.e., Channa stewartii, C. striatus and Labeo gonius of Assam.      

Methodology: Three fish species, namely Channa stewartii, C. striatus and Labeo gonius were collected from two beels, i.e., Thekera and Samaguri beels of Assam, India. Standard procedures were followed for isolation of their DNA and sequencing. Thereafter, genetic distance and diversity and phylogenetic studies of three species were calculated using computer based softwares ClustalW and MEGA6.???     

Results: In the present study, the pairwise genetic distance of three fish species ranged from 0 to 20.436. No pairwise distance was found between two stocks at different locations Thekera and Samaguri beels of Assam for each of three species C. stewartii, C. striatus and L. gonius. The highest values (20.436) of pairwise distance were found between C. striatus and L. gonius population of Thekera beel; Thekera and Samaguri beels; and L. gonius population of the Thekera and Samaguri beels. The overall average distance for the two populations of three fish species was 15.387. No significant intraspecific difference was observed in the phylogenetic studies of two stocks of three fish species. ?? 

Interpretation: The overall average distance (15.387) of three fish species could be attributed to genetic distance from each other. The high value of coefficient of differentiation (1.000) for three fish species from two stocks indicated that all the species were different from each other. Overall genetic and phylogenetic studies revealed that C. stewartii, C. striatus and L. gonius. were less differentiated from each other.

Key words: Barcoding, Channa stewartii, C. striatus, Labeo gonius, Phylogenetic relationship

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