JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP
About Journal
    Home
    Obituary: Dr. R. C. Dalela
    Editorial Board
    Reviewer Panel
    Publication Policies
    Guidelines for Editors
    Guidelines for Reviewers
    Abstracting and Indexing
    Subscription and Payments
    Contact Journal
    About Triveni Enterprises
 
Read Journal
    Current Issue
    Journal Archives
 
For Authors
    Guidelines for Authors
    Terms and Conditions
    Author Resources
    Fees and Payments
    Track Paper Status
 

Google Search the Journal web-site:


    Abstract - Issue Nov 2019, 40 (6)                                     Back


nstantaneous and historical temperature effects on a-pinene

Antibacterial activity of Eriobotrya japonica leaf extracts against methicillin-resistant Staphylococcus aureus

 

Paper received: 27.12.2018??????? ?????????????????????????????????????????????????????????? Revised received: 25.03.2019????? ???????????????????????????????????????????????? Accepted: 18.04.2019

 

 

Authors Info

D. Yu1, I.H. Yun2, N.T.D. Pham2, Y.M. Jang2, S.K. Park2,

S.H. Eom3 and Y.M. Kim2,4*     

  

1Institute of Food Industrialization,

Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang 25354, Korea

 

2Department of Food Science and

Technology, Pukyong National

University, Busan 48513, Korea

 

3Department of Food Science

and Technology, Dongeui

University, Busan 47340, Korea

 

4Marine-Integrated Bionics

Research Center, Pukyong National University, Busan 48513, Korea

 

    

*Corresponding Author Email :

ymkim@pknu.ac.kr

 

 

 

Abstract

 

Aim: To investigate antimethicillin-resistant Staphylococcus aureus (MRSA) activity of E. japonica leaf and to elucidate its anti-MRSA mechanism.    

 

Methodology: Methanolic extract of dried E. japonica leaf was partitioned in n-hexane, dichloromethane, ethyl acetate (EtOAc) and n-butyl alcohol. Antibacterial activity of extracts was determined by disk diffusion assay and minimum inhibitory concentration.Total RNA isolation was performed by treating MRSA culture with EtOAc sub-fractions. All MRSA isolates were tested for the presence of genes by reverse transcription polymerase chain reaction, followed by Western Blot.    

 

Results: The highest MRSA activity was observed from EtOAc sub-fraction 03 of methanolic extract. The minimum inhibitory concentration values of EF03 ranged from 32 to 64 ?g ml-1 against methicillin-susceptible and -resistant S. aureus. EF03 fractions inhibited the expression of resistant genes in a dose-dependent manner. Moreover, the results of Western Blot assay indicated that the EF03 fractions inhibited the expression levels of resistant protein, PBP2a in a dose-dependent manner.    

 

Interpretation: EtOAc soluble fraction of E. japonica leaf evidenced profound antimicrobial activity, and inhibited expression of resistant genes against MRSA.

 

Key words: Antibacterial activity, Eriobotrya japonica, MRS A activity, Synergistic effect

 

 

 

Copyright ? 2019 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).