Journal of Environmental Biology

Production of antimicrobial adhesives 

Paper received: 30.10.2018???????????????????????????????????????????????????????????? Revised received: 06.03.2019????????????????????????????????????????????????????????????? Accepted: 16.03.2019

Authors Info

D. Yuvaraj*, R. Gnanasekaran,

J. Iyyappan, I. Subashini,

S. Nandhini, M. Jayasudha,

R. Shaleni and M. Shyam

Department of Biotechnology, Vel Tech High Tech Dr. Rangarajan Dr. Sakunthala Engineering College, Avadi, Chennai-600 062, India

*Corresponding Author Email :

yuvarajdinakarkumar@gmail.com

Abstract

Aim: To produce a bio mimetic consortium embedded with chitosan, haemolymph and calcium nanoparticles to enhance its activity in wound healing.    

Methodology: Haemolymph was collected with a syringe from the abdominal region of the snails. Nanoparticles were prepared from the powdered samples of shells and operculum by addition of HCl and NaOH. Later, it was calcinated in the muffle furnace. SEM analysis was performed to check for the formation of nanoparticles. To extract chitosan, the cockroaches were dried in hot air oven, then crushed and powdered. By the process of de-protenization and de-mineralization, chitin was obtained by de-acetylation process to produce chitosan. Haemolymph was checked for anti-bacterial property which was determined by agar dilution method.

Results: The results obtained indicated that the consortium prepared from the combination of calcium nanoparticles, chitosan and haemolymph proved to have an impact on the growth of Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Bacillus licheniformis. These synthesized compounds has individual potency too.    

Interpretation: All the samples, haemolymph, calcium nanoparticles and chitosan showed anti-microbial property and were embedded in the bandages. Hence, the wound healing property of bandages were increased and its activity was verified.

Key words: Antibacterial activity, Bandages, Calcium nanoparticles, Chitosan, Haemolymph

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