Journal of Environmental Biology

Degradation of hydrocarbon by isolated fungal species with

laccase activity

Paper received: 30.10.2018???????????????????????????????????????????????????????????? Revised received: 06.03.2019????????????????????????????????????????????????????????????? Accepted: 16.03.2019

Authors Info

R. Gnanasekaran, J. Iyyappan*, S. Deepeka, R. Mageshwari,

S. Saranraj and

V. Sathayanarayanan

Department of Biotechnology, Vel Tech High Tech Dr. Rangarajan Dr. Sakunthala Engineering College, Chennai ? 600 062, India

*Corresponding Author Email :

jiyyappan.biotek@gmail.com

Abstract

Aim: Hydrocarbon of crude oil and other oil products such as gasoline comprise aromatic, aliphatic and alicylic components pollute water bodies are hazardous. Microbial degradation of these oil spills are an efficient way. Degrading oil contamination by microorganism is the theme of this paper. To study degrading efficiency of crude oil hydrocarbon by fungi.    

Methodology: Fungal species were isolated from crude oil soaked soil samples and oil contaminated soil samples from commercial oil industry. Isolated fungal species were cultured on nutrient media such as potato dextrose agar (PDA) and czapekdox agar at 37 ?C for 7 day. Further, fungal species were identified by 18 S rRNA sequencing. The potential of fungi to degrade hydrocarbon was investigated through laccase and peroxidase. To examine the ability to degrade oil, the fungal species were screened on Czapekdox agar and broth medium containing petroleum derivatives. Laccase activity was performed by ABTS (2, 2̍-azinobis 3-ethyl-benzothiazoline-6-sulphonate) and bromophenol. They were used as substrate for screening laccase activity.    

Results: Among the isolated eight fungal species, isolate FGS -4 showed better biodegradation ability and subsequent 18 S rRNA revealed that isolate FGS-4 belongs to Aspergillus tubigensis. Production of laccase enzyme was confirmed. The results of gas chromatography analysis showed that hydrocarbon was biodegraded by the isolated Aspergillus tubigensis.    

Interpretation: Biodegradation of hydrocarbon by Aspergillus species would create an impact on various waste treatment methods. Eco-friendly degradation of hydrocarbons could be achieved by Aspergillus species.

Key words: Bioremediation, Fungal species, Hydrocarbons, Laccase activity

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