Journal of Environmental Biology

Studies on potential application of crude keratinase enzyme from Stenotrophomonas sp. for dehairing in leather processing industry

C. Swornakumari, S. Meignanalakshmi*, R. Legadevi and A. Palanisammi

Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 007, India

*Corresponding Author E-mail: smeignanalakshmi@gmail.com

Key words

Controlled drug release

Drug encapsulation

Gentamicin

Poly-3-hydroxybutyrate

Publication Data

Paper received : 06.09.2016

Revised received : 25.11.2016

Re-revised received : 04.08.2017

Accepted : 10.10.2017?  

Abstract

Aim: Biodegradable poly-3-hydroxybutyrate is one of the most common biopolymer which is used in different fields such as medicine, agriculture, textile, industrial and food packaging. PHB microspheres are useful for targeting drugs to specific infection sites and for prolonged drug release. The present study focus on microsphere preparation for effective controlled drug release using poly-3-hydroxy butyrate biopolymer.  

Methodology: In the present study, poly-3-hydroxy butyrate microspheres were prepared using solvent evaporation technique and characterized by SEM and FTIR. Poly-3-hydroxy butyrate microspheres were encapsulated with BSA and gentamicin. Drug encapsulation efficiency was determined. In vitro drug release profile and in vitro cytotoxicity was also studied.

Results: The prepared microspheres were of 2?m in size. Microspheres fabricated with 1% polyvinylalcohol showed encapsulation efficiency of 94.3% and 90.27% with BSA and gentamicin, respectively. The in vitro release studies in simulated body fluid, phosphate buffered saline and contact lens solution showed initial burst release followed by controlled release. In vitro cytotoxicity analysis showed 98% and 95% viability of cells in 3T3L1 cell line for microsphere encapsulated with BSA and gentamicin, respectively. ?     

Interpretation: Poly-3-hydroxy butyrate microspheres were found to release BSA and gentamicin in a controlled manner and were found to be non-toxic by in vitro cytotoxicity studies.

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