Journal of Environmental Biology

Efficacy of amylase for wastewater treatment from Penicillium sp. SP2 isolated from stagnant water

F. S. Priya¹* and A. Renu²

¹Department of Biochemistry, Sathyabama University, Chennai- 600 119, India

²Department of Biotechnology, Udaya School of Engineering, Kanyakumari-629 204, India

*Corresponding Author E-mail: starletbiochemist@gmail.com

 Key words

Amylase toxicity

Jack fruit seed

Penicillium sp. SP2

Solid substrate fermentation

Publication Data

Paper received : 18.10.2016

Revised received : 28.01.2017

Re-revised received : 25.04.2017

Accepted : 27.06.2017

Abstract

Aim: Amylolytic enzymes are useful in bakery, food, automation dishwashing, fodder, wastewater treatment and poultry. The main objective of the present study was to utilize jack fruit seed as a cheap substrate for the production of amylase in solid substrate fermentation using Penicillium sp. SP2 and to treat wastewater.

Methodology: Stagnant water was used to isolate Penicillium sp. SP2 for amylase production. The potent fungal isolate, Penicillium sp. SP2 was cultured in solid substrate fermentation using jack fruit seed as a low cost substrate. The process parameters were optimized to increase the production of enzyme. Amylase was initially fractionated using ammonium sulphate and purified using sephadex G-75 gel filtration column. The crude amylase from Penicillium sp. SP2 was applied to treat wastewater.

Results: The isolated Penicillium sp. SP2 utilized jack fruit seed substrate and produced amylase. Among the carbon sources, glucose significantly increased the production of enzyme. Beef extract increased amylase production than other nitrogen source. Ca²⁺ ion also significantly enhanced the production of amylase. Amylase production was found to be maximum at 35 ^?C. The molecular weight of purified amylase was 57 kDa as judged by SDS - PAGE. The crude enzyme reduced carbohydrate content in wastewater and increased dissolved oxygen considerably.? ?   

Interpretation: Considering cheap cost, availability, and nutritive value, jack fruit seed is a novel substrate for amylase production for various biotechnological applications. The amylase from Penicillium sp. SP2 find great application in wastewater treatment.

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