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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP
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    Abstract - Issue Mar 2018, 39 (2)                                     Back


nstantaneous and historical temperature effects on a-pinene

Molecular characterization and genetic diversity analysis of

selected maize inbreds using SSR markers

 

T. Sharma1,2*, A. Kumar3,4, S. C. Dwivedi1 and R. P. Vyas2

1School of Biosciences, Suresh Gyan Vihar University, Jaipur-302 017, India

2Department of Genetics and Plant Breeding, CSAUAT, Kanpur-208 002, India

3UP Council of Agricultural Research (UPCAR), Lucknow- 226 010, India

4Department of Botany, Ch. Charan Singh (PG) College, Heonra (Saifai), Etawah- 206 002, India

*Corresponding Author E-mail: trivenidevsharma@gmail.com

 

 

 

 

Key words

Cluster analysis

Genetic diversity

Molecular characterization

Zea mays

SSR markers 

 

 

Publication Data

Paper received : 30.06.2016

Revised received : 04.05.2017

Re-revised received : 15.06.2017

Accepted : 29.08.2017

 

Abstract

Aim: The aim of the current study was to analyze the molecular diversity and genetic relationship among selected maize inbreds using SSR markers.

 

Methodology: Genomic DNA of individual inbreds was isolated by using the standard CTAB procedure (Doyle and Doyle, 1987) with some modifications. Thermal cycler was used to perform the polymerase chain reactions. 50ng of genomic DNA was used in 10 ?l total volume with a final concentration of 10X Taq buffer, MgCl2 (50 ?M), each primers (10 ?M), nucleotides (2.5 mM) and 1 U Red Taq DNA polymerase. Amplified PCR products were resolved by electrophoresis on horizontal gel (3.0% Agarose gel) system using 1x TBE Buffer. The gels were photographed with CCD camera attached to a gel documentation system.

 

Results: In total, 191 alleles were detected which showed a range of 2 to 6 alleles per marker and an average value of 3.82 alleles per locus. Polymorphic information content (PIC) value showed a range of 0.050 (umc1069) to 0.817 (bnlg1823) with an average value of 0.585 indicating the efficiency of markers to study the level of polymorphism available in the maize inbred lines. The value of genetic similarity coefficients for the twenty maize inbreds based on 50 SSR markers ranged from 0.51 to 0.74. The maximum value of similarity coefficient was observed between CIM-127 vs CIM-130, whereas the minimum value of it was between TSK-7 vs TSK-2, TSK-41 vs TSK-2. Cluster analyses, done using matrices of similarity in the NTSYS-pc software, classified studied inbred lines into four distinct clusters, Cluster A, B, C and D comprising of 5, 5, 5 and 5 maize inbred lines, respectively. ???   

 

Interpretation: In this study, PIC values indicated a good efficiency of markers for studying the polymorphism level available in studied inbred lines. High level of diversity among the inbreds detected with SSR markers indicated their suitability for further breeding programs.

 

 

 

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