Journal of Environmental Biology

Role of biochemical constituents and minerals

against cotton leaf curl disease in cotton

S. Mandhania*¹, R.S. Sangwan¹, S.S. Siwach², S.R. Pundir¹, O. Sangwan¹ and A. Janu³

¹Cotton Section, Department of Genetics and Plant Breeding, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125 001, India

²Directorate of Research, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125 001, India

³Department of Entomology, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125 001, India

*Corresponding Author E-mail: smbiochem@gmail.com

Key words

Cotton genotype

Leaf curl disease

Phenols

Polymerase chain reaction

Viral infection 

Publication Data

Paper received : 10.05.2016

Revised received : 22.11.2016

Re-revised received : 20.05.2017

Accepted : 24.06.2017

Abstract

Aim: Cotton Leaf Curl Disease (CLCuD) is among the most devastating disease infecting the cotton plants. The plants have developed both inherent, consecutive and induced defence mechanism against the disease like pre-existing physical and chemical barriers, such as inducible defence responses that interfere with pathogen establishment. Therefore, the present study was conducted to examine the status of biochemical constituents and minerals with their imparting defensive role against CLCuD.

Methodology: The four cotton genotypes H 1478, H 1098i, H 1156 and H 1463 were screened for cotton leaf curl disease on the consecutive and induced defensive role basis of polyphenoloxidase, peroxidase, biochemical constituents and minerals. The genotypes were grown under field condition at Cotton Research Farm, Chaudhary Charan Singh Haryana Agricultural University, Hisar. Leaf samples of healthy and diseased cotton plants were collected and analyzed for biochemical constituents, minerals and enzymes activities.

Results: The amount of phenol, gossypol and soluble protein were significantly higher in healthy plant as compared to diseased plant in all genotypes, whereas sugar content differed non-significantly and inconsistently among the genotypes. The polyphenoloxidase and peroxidase activities were also found significantly higher in healthy plant in all genotypes and correlate with high amount of phenolic compounds. Potassium, manganese, zinc and sulphur were significantly less in diseased plant of all genotypes. Inconsistent pattern was observed among genotypes in response to CLCuD for copper content. It differed significantly between healthy and diseased plants only in H 1156 and H 1463 genotypes. ???   

Interpretation: The high content of potassium, manganese, sulphur and phenolic compounds in healthy plant of H 1463 genotypes leads to path for plant protection by means of their direct and indirect role against plant pathogen.

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