JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP
About Journal
    Home
    Obituary: Dr. R. C. Dalela
    Editorial Board
    Reviewer Panel
    Publication Policies
    Guidelines for Editors
    Guidelines for Reviewers
    Abstracting and Indexing
    Subscription and Payments
    Contact Journal
    About Triveni Enterprises
 
Read Journal
    Current Issue
    Journal Archives
 
For Authors
    Guidelines for Authors
    Terms and Conditions
    Author Resources
    Fees and Payments
    Track Paper Status
 

Google Search the Journal web-site:


    Abstract - Issue Mar 2018, 39 (2)                                     Back


nstantaneous and historical temperature effects on a-pinene

Identification of desirable mutants in quantitative traits

of lentil at early (M2) generation

 

D. Tabti, M. Laouar1, K. Rajendran, S. Kumar and A. Abdelguerfi

1ENSA, Laboratoire d'Am?lioration Int?grative des Productions V?g?tales, Alger, 16051, Alg?rie

2ENSA, Laboratoire des Ressources G?n?tiques et Biotechnologies, Alger, 16051, Alg?rie

3Biodiversity and Integrated Gene Management Program. The International Centre for Agricultural Research in Dry Areas, Rabat-Institutes,10020, Morocco

*Corresponding Author E-mail: tdahbia1990@gmail.com

 

 

 

Key words

BLUPs values

Gamma rays

Lentil

Lethal dose

Mutants

 

 

 

Publication Data

Paper received : 05.10.2016

Revised received : 07.02.2017

Re-revised received : 30.05.2017

Accepted : 28.06.2017

 

Abstract

Aim: Narrow genetic base and limited genetic diversity are the major research constraints that affect the efficacy of class breeding methods in lentil. In order to circumvent these conditions, mutation breeding techniques were successfully employed in many studies. The present study was conducted to find the LD50 dose of gamma rays in lentil cultivar, Idlib-3 and also to develop and characterize M2 population for economically important quantitative traits.

 

Methodology: Initially, seeds of lentil variety Idlib-3 were treated with eight doses (45, 60, 75, 90, 100, 200, 300 and 400 Gy) of gamma rays. Germination percentage was recorded to determine LD50 by probit analysis. After determining LD50, M1 and M2 generations were raised. At M2 generation, mutant families were characterized for a set of ten different economically important traits.

 

Results: The LD50 of gamma rays was calculated as 104.34 Gy based on the germination percentage. In M2 generation many induced mutations such as chlorophyll mutation (2.76%), stunted growth (1.14%) and dwarf mutants (0.35%) were recorded. Dunnett's test revealed a total of 13 superior families over parent for various quantitative traits. The results of Best Linear Unbiased Predictors (BLUPs) confirmed the recurrence and superiority of same seven families, identified in Dunnett's test for high seed yield. ? 

 

Interpretation: Early selection in M2 generation was found useful to identify new desirable mutant traits in lentil. The superior families identified for early maturity, high yield and more total number of pods per plants could be either utilized as direct mutants or in the future crossing program.

 

 

Copyright ? 2018 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).