Journal of Environmental Biology

Determining the genetic relationships between cultivated type olives using ISSR and morphological markers

E.D. Yegenoglu¹*, M. Sesli² and Y. Gevrekci³

¹Department of Plant and Animal Production, Alasehir Vocational School, Manisa Celal Bayar University, Alasehir, Manisa, 45400, Turkey

² Department of Tobacco Breeding, School of Tobacco Expertise, Manisa Celal Bayar University, Akhisar, Manisa, 45210, Turkey

³Department of Animal Science, Unit of Biometry-Genetics, Bornova, Agriculture Faculty, Ege University, Izmir, 35100, Turkey

*Corresponding Author E-mail: yegenoglu75@hotmail.com

Key words

Euclidean distance,

ISSR markers,

Morphological characters,

Olives,

UPGMA

Publication Data

Paper received : 08.08.2016

Revised received : 25.06.2017

Accepted : 28.06.2017

Abstract

Aim: The study aimed to compare morphological characters and Inter-simple sequence repeat (ISSR) data based trees, and examine the genetic relations in ten olive varieties among cultivated type olives grown commonly in different regions of Turkey.

Methodology: Ten olive varieties were evaluated with some morphologic markers and ISSR marker. All analyses were conducted with Numerical Taxonomy System (NTSYS). The cluster analysis was performed with unweighted pair group method with arithmetic average (UPGMA) clustering algorithm.

Results: The results showed that there was a moderate correlation between pairwise distances estimated from ISSR data and distances from morphological characters (0.511). The Euclidean Distance matrix represented that the lowest value was between Tavsan Yuregi and Cilli (1.62), while the highest value was between Manzanilla and Cekiste (7.91). According to Jaccard coefficient, the samples closest to each other were (Memecik and Gemlik); and the samples farthest to each other were (Halhali and Manzanilla).

Interpretation: Determining the genetic relations in agriculturally economic plants is valuable in terms of protecting the gene sources, determining the homonyms and synonyms, and developing breeding programs. Morphological and molecular markers may be used in the identification of genetic variability. Mutually complementary information can be obtained by using morphological and molecular markers together.

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