Journal of Environmental Biology

Physiological properties of Engraulis japonicus muscle protein hydrolysates prepared by subcritical water hydrolysis

J.S. Choi^1,2, D.B. Jang¹, H.E. Moon¹, M.K. Roh³, Y.D. Kim⁴, K.K. Cho⁵ and I.S. Choi^1,6*

¹RIS Center, Industry-Academic Cooperation Foundation, Silla University, Busan-46958, Republic of Korea

²Division of Bioindustry, College of Medical and Life Sciences, Silla University, Busan-46958, Republic of Korea

³Department of R&D, Korea Bio & Engineering Co. Ltd., Nam-Gu, Busan-48434, Republic of Korea

⁴National Institute of Fisheries Science, Sanyang-Eup, Tongyeong-Si, Gyeongsangnam-Do-53085, Republic of Korea

⁵Department of Animal Resources Technology, Gyeongnam National University of Science and Technology, Gyeongnam-52725, Republic of Korea

⁶Department of Life Science, Silla University, Busan-46958, Republic of Korea

*Corresponding Author E-mail: ischoi@silla.ac.kr

Key words

Antioxidant activity,

Engraulis japonicus,

Hydrolysate,

Subcritical water hydrolysis,

Tyrosinase inhibitory activity

Publication Data

Paper received: 14.03.2015

Revised received: 14.07.2016

Accepted: 12.09.2016

Abstract

Aim: To evaluate the beneficial biological activities of Engraulis japonicus muscle protein, the antioxidant and tyrosinase inhibitory activities of E. japonicus muscle protein hydrolysates prepared by subcritical water hydrolysis were investigated.

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Methodology: To evaluate the bioactivity of E. japonicus subcritical hydrolysates, the applied temperature (pressure) was 140?C (2.6 bar); the reaction times were 0, 5, 10, 15 and 20 min.

Results: After 10 min of subcritical hydrolysis of particles obtained using 80-200 mesh, 46.39% of the E. japonicus muscle protein was hydrolyzed at 140?C. According to the response surface methodology results, particles generated with 80-200 mesh E. japonicus powder exhibited an increased yield of hydrolysates. The highest DPPH-radical-scavenging activity (34.91%) occurred in 60-80-mesh sized protein hydrolysates treated at 140?C for 15 min, and the highest tyrosinase inhibitory activity (99.24%) was identified in 80-200-mesh sized protein hydrolysates treated at 140?C for 5 min. Changes in the molecular weight distribution of E. japonicus muscle proteins after subcritical water hydrolysis were assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. ????

Interpretation: Subcritical water hydrolysis is a suitable technique for generating E. japonicus muscle protein hydrolysates with useful biological activities, within a short (5?15 min) time frame.

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