Journal of Environmental Biology

Immobilization of laccase onto micro-emulsified magnetic nanoparticles for enhanced degradation of a textile recalcitrant

Nagarajan Balaji¹, Kannaiyan Sathish Kumar²,*, Muthulingam Seenuvasan³, Govindasamy Vinodhini¹ and Madhava Anil Kumar¹

 ¹Department of Biotechnology, Madha Engineering College, Chennai-600 069, India

²Department of Chemical Engineering, SSN College of Engineering, Chennai-603 110, India

³Department of Petrochemical Engineering, SVS College of Engineering, Coimbatore-642 109, India

*Corresponding Author E-mail: sathishkannaiyan.k@gmail.com

Publication Data

Paper received:

20 September 2015

Revised received:

12 December 2015

Re-Revised received:

16 May 2016

Accepted:

26 August 2016

Abstract

The present investigation evaluates the efficiency of laccase binding onto the micro-emulsified magnetic nanoparticles (MNPs). The synthesized MNPs were characterized for its nature, particle size and functional moieties using transmission electron microscopy Fourier transform infrared spectroscopy and X-ray diffraction studies. It was found that the MNPs had a uniform and mean average diameter of 12.63?3.2 nm. The TEM analysis and XRD patterns also confirmed the presence of MNPs after laccase binding. The maximum activity of the immobilized laccase was obtained at its weight ratio of 19.55x10^-3 mg bound laccase mg^-1 MNPs. The stability and reusability analyses were also studied to evaluate the activity of the immobilized laccase with that of free laccase. Application of laccase immobilized MNPs were extended to degrade a reactive textile dye; reactive green 19A (RG19A).   

 Key words

Laccase, Micro-emulsion, Magnetic nanoparticles, Reactive green 19A

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