Journal of Environmental Biology

Real time PCR expression analysis of gene encoding p5cs enzyme and proline metabolism under NaCl salinity in rice

D.L. Bagdi*, B.P. Shaw, B.B. Sahu and G.K. Purohit

Laboratory of Translational Research and Technology Development, Institute of Life Sciences, Bhubaneswar-751 023, India

*Corresponding Author?s Email : dlbagdi.2000@gmail.com

Publication Data

Paper received:

20 September 2013

Revised received:

10 March 2014

Re-revised received:

20 May 2014

Accepted:

05 March 2015

Abstract

Regulation of proline accumulation in seedlings of rice (Oryza sativa L. cv. Lunishree) was investigated. The increasing concentration of NaCl from 85 mM to 425 mM NaCl progressively increased proline content in rice. The maximum increase in proline content was recorded at 425 mM NaCl concentration as compared to control and other concentrations of NaCl. The highest significant activity of proline synthesizing enzymes, Δ¹-Pyrrolline-5-carboxylate synthetase, Δ¹-Pyrrolline-5-carboxylate reductase and Ornithine-δ-aminotransferase with lowest activity of proline hydrolysis enzymes;Proline dehydrogenase was also recorded at 425 mM NaCl salinity over control and other concentrations of NaCl with insignificant increase in the activity of Δ¹-Pyrrolline-5-carboxylate synthetase and Ornithine-δ-aminotransferase at 85 mM NaCl over control.It was found that the transcript of gene encoded with p5cs is up regulated about 1.35 folds under salinity stress. This gene synthesis an osmo protectant to help the plant resist the change in osmotic imbalances. Externally addition of MnCl2 at 300mg/220 ml ? strength Hoagland solution, having 1% NaCl, was also seen to increase 893.9 % proline content of this variety as compared to control.     

 Key words

OAT, P-5-CR, P-5-CS gene, P-5-CS, PDH, Proline, Rice, RT-PCR 

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