Journal of Environmental Biology

Screening of different Trichoderma species against agriculturally important foliar plant pathogens

Narayanasamy Prabhakaran^1,2, Thokala Prameeladevi¹*, Muthukrishnan Sathiyabama² and Deeba Kamil¹

¹Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi-110 012, India.

²Department of Plant Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli - 620 024, India

*Corresponding Author E-mail: prameelancha@yahoo.co.in

 Publication Data

Paper received:

21 October 2013

Revised received:

15 March 2014

Accepted:

20 March 2014

Abstract

Different isolates of Trichoderma were isolated from soil samples which were collected from different part of India. These isolates were grouped into four Trichoderma species viz.,?? Trichoderma asperellum (Ta), T. harzianum (Th), T. pseudokoningii (Tp) and T. longibrachiatum (Tl) based on their morphological characters. Identification of the above isolates was also confirmed through ITS region analysis. These Trichoderma isolates were tested for in vitro biological control of Alternaria solani, Bipolaris oryzae, Pyricularia oryzae and Sclerotinia sclerotiorum which cause serious diseases like early blight (target spot) of tomato and potato, brown leaf spot disease in rice, rice blast disease, and white mold disease in different plants. Under in vitro conditions, all the four species of Trichoderma (10 isolates) proved 100% potential inhibition against rice blast pathogen Pyracularia oryzae. T. harzianum (Th-01) and T. asperellum (Ta-10) were effective with 86.6% and 97.7%, growth inhibition of B. oryzae, respectively. Among others, T. pseudokoningii (Tp-08) and T. longibrachiatum (Tl-09) species were particularly efficient in inhibiting growth of S. sclerotiorum by 97.8% and 93.3%. T. longibrachiatum (Tl-06 and Tl-07) inhibited maximum mycelial growth of A. solani by 87.6% and 84.75. However, all the T. harzianum isolates showed significantly higher inhibition against S. sclerotiorum (CD value 9.430), causing white mold disease. This study led to the selection of potential Trichoderma isolates against rice blast, early blight, brown leaf spot in rice and white mold disease in different crops.  

 Key words

Antagonistic activity, Biocontrol, Dual culture technique, Foliar plant pathogens, Trichoderma species

Copyright ? 2015 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).