Journal of Environmental Biology

Application of response surface methodology for optimization of polygalacturonase production by Aspergillus niger

Kaushlesh K. Yadav1, Neelima Garg¹*, Devendra Kumar¹, Sanjay Kumar¹, Achal Singh² and M. Muthukumar³

¹Division of Post Harvest Management, Central Institute for Subtropical Horticulture, Rehmankhera, Lucknow?226 101, India

²Division of Crop Production, Central Institute for Subtropical Horticulture, Rehmankhera, Lucknow?226 101, India

³Division of Crop Improvement and Biotechnology, Central Institute for Subtropical Horticulture, Rehmankhera, Lucknow?226 101, India

*Corresponding Author E-mail: neelimagargg@rediffmail.com

Publication Data

Paper received:

05 May 2013

Revised received:

22 December 2013

Accepted:

03 March 2014

Abstract

Polygalacturonase (PG) degrades pectin into D-galacturonic acid monomers and is used widely in food industry especially for juice clarification. In the present study, fermentation conditions for polygalacturonase production by Asgergillus niger NAIMCCF-02958, using mango peel as substrate, were optimized using the 2³ factorial design with central composite rotatable experimental design (CCRD) of response surface methodology (RSM). The maximum PG activity 723.66 U g^-1 was achieved under pH 4.0, temperature 30 ?C and 2% inoculum by response surface curve. The experimental value of PG activity was higher 607.65 U g^-1 than the predicted value 511.75 U g^-1. Under the proposed optimized conditions, the determination coefficient (R²) was equal to 0.66 indicating that the model could explain 66% of the total variation as well as establish the relationship between the variables and the responses. ANOVA analysis and the three dimensional plots also confirmed interactions among the parameters. ?  

 Key words

Aspergillus niger, Enzyme production, Mango peel, Polygalacturonase production, Response surface methodology 

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