Journal of Environmental Biology

Isolation and characterization of fluorescent pseudomonads and their effect on plant growth promotion

G.? Anitha and B.S. Kumudini*

Department of Biotechnology, Center for Post Graduate Studies, Jain University, Jayanagar, Bangalore ? 560 011, India

*Corresponding Author E-mail: kumudini_satyan@yahoo.co.in

Publication Data

Paper received:

20 November 2012

Revised received:

09 May 2013

Re-revised received:

02 August 2013

Accepted:

13 August 2013

Abstract

Seven isolates of fluorescent pseudomonads were evaluated for their effect on plant growth promoting traits, both under normal and saline conditions using tomato plants. Fifteen rhizosphere samples from crop fields' of rice, chilly, ragi, beans and garden soils from different regions of India were collected and used for further study. They were characterized morphologically and biochemically which led to a conclusion that they may belong to genus Pseudomonas. They were also analyzed for their plant growth promoting activities such as production of indole acetic acid, siderophore, hydrogen cyanide and ammonia. It was observed that all the isolates were able to produce these compounds, but to varying extent.? But, isolate JUPF37 produced highest followed by JUPF32. Study showed that out of seven isolates of fluorescent pseudomonads, JUPF37 showed highest plant growth promoting traits both under normal and saline conditions. ??

 Key words

Fluorescent pseudomonads, Indole acetic acid, PGPR, Plant growth promotion, Siderophore

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