Journal of Environmental Biology

Aerobic biodegradation of azo dye Acid Black-24

by Bacillus halodurans 

A.S. Arun Prasad and K.V. Bhaskara Rao*

Division of Environmental Biotechnology, School of Biosciences and Technology, VIT University, Vellore-632 014, India

*Corresponding Author E-mail: kokatibhaskar@yahoo.co.in

 Publication Data

Paper received:

12 September 2012

Revised received:

20 June 2013

Re-revised received:

16 August 2013

Accepted:

19 August 2013

Abstract

Bacillus halodurans MTCC 865 was employed for decolorization of textile azo dye, Acid Black-24 (AB-24). Thousand mgl^-1of AB-24 was decolorized with 90% efficiency by the strain within 6 hrs at pH 9 and 37?C with 5% NaCl under static conditions in screening medium. Decolorization was evaluated by measuring the periodic decrease in absorbance at 557nm (λ_max). Biodegradation of Acid Black-24 was determined by FTIR and HPLC. The FTIR spectrum of the AB-24 dye suggests the presence of azo bond (?N=N?) peak at 1618.28 cm^?1. Absence of the azo bond in the degraded sample spectrum indicates biodegradation of the dye. Formation of metabolites with different retention times in HPLC analysis further confirmed degradation of the azo dye, Acid Black-24 by Bacillus halodurans. ? 

 Key words

Acid black-24, Bacillus halodurans MTCC 865, Biodegradation, Decolorization 

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