Journal of Environmental Biology

Profiling of the dichloromethane-induced

proteome expression changes

Seul-Ki Park and Mi-Young Lee*

Department of Medical Biotechnology, SoonChunHyang University, Asan, Chungnam 336 600, Korea

*Corresponding Author E-mail: miyoung@sch.ac.kr

Publication Data

Paper received:

25 May 2012

Revised received:

08 November 2012

Accepted:

24 December 2012

Abstract

A colorless volatile liquid dichloromethane (DCM) is used as solvents in chemical manufacturing processes. The major route of exposure is via inhalation and to a lesser extent through the skin and digestive tract. We investigated the effects of DCM on rats and analyzed their liver proteome expression changes. Approximately 1,100 protein spots that were detected by 2-dimensional gel electrophoresis showed reproducible abundance. Mass spectrometry based proteomics was used to characterize the changes in the liver proteome in response to DCM exposure. Consequently, 7 of these spots showed significant changes in expression level after DCM treatment. These proteins were 3 paralogues of glutathione S-transferase, beta 1 globin, 2 hemoglobin beta-2 and alpha-2 globulin. Of these, the expression of alpha-2 globulin was also confirmed by western blot. The differential expression of these proteins might be caused by DCM exposure.

 Key words

Dichloromethane, Liver protein, Proteomics, Rat

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