Lactic
acid production from cassava fibrous residue using
Lactobacillus plantarum
MTCC 1407
Ramesh Chandra Ray*, Piyush Sharma and Smita Hasini
Panda
Regional
Centre of Central Tuber Crops Research Institute, Bhubaneswar
- 751 019,? India
(Received:
October 25, 2008;
Revised received: July
09, 2008; Accepted: July 29, 2008)
Abstract: During
extraction of starch from cassava, fibrous residue is a major waste released
into the environment.
Owing to the high starch content (60- 65% on dry weight basis) and organic
matter of cassava fibrous residue (CFR), an attempt has been made to utilize it
for the production of lactic acid (LA) in semi- solid state fermentation using
Mann Rogassa Sharpe medium containing [5 % (wv-1)] CFR in lieu of
glucose [2 % (wv-1)] as the carbon source. Response Surface
Methodology (RSM) was used to evaluate the effect of main variables, i.e.
incubation period, temperature and pH on LA production. The experimental
results showed that the optimum incubation period, temperature and pH were 120
hr, 350C and 6.5, respectively. Maximum starch conversion by
Lactobacillus plantarum MTCC 1407 to LA was 63.3%. The organism produced 29.86
g of (L+) LA from 60 g of starch present in 100? g of CFR. The LA production yield
(i.e. mass LA produced mass starch present in CFR-1 x 100) was 49.76 %.
Key words: Cassava fibrous residue, Lactobacillus plantarum MTCC 1407,? Lactic acid,
Response
surface methodology, Semi- solid fermentation
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