Journal of Environmental Biology

The influence of developmental stages and protective additives on
cryopreservation of surf clam (Spisula sachalinensis) larvae

Youn Hee Choi¹, Jeong Yong Lee² and Young Jin Chang^*3

¹Institute of Fisheries Sciences, Pukyong National University, Nam-gu, Busan 608-737, Korea
²National Fisheries Research and Development Institute, Gijang-gun, Busan 619-902, Korea
³Department of Aquaculture, Pukyong National University, Nam-gu, Busan 608-737, Korea

(Received: March 13, 2007; Revised received: December 28, 2007; Accepted: December 31, 2007)

Abstract: This study was performed to find out the optimal larval stage and the most desirable protective additives for cryopreservation of surf clam, Spisula sachalinensis larvae. The survival rates of frozen-thawed larvae increased with post developmental stage. The highest value of 96.1±1.0% was achieved using umbo stage larva as developmental stage and 0.2 M sucrose as protective additive.

Key words: Surf clam, Developmental stage, Protective additive, Cryopreservation

PDF of full length paper is available with author (yjchang@pknu.ac.kr)

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