Journal of Environmental Biology

Isolation of cadmium-induced DNA sequence in microalga Nannochloropsis oculata

Joong Kee Min², Tae Yeon Rhim², Hyun Woong Shin¹ and Mi Young Lee^*2

¹Department of Marine Biotechnology, Soonchunhyang University, Asan, Chungnan - 336-600, Korea
²Department of Biotechnology, Soonchunhyang University, Asan, Chungnam - 336-600, Korea

(Received: March 22, 2007; Revised received: November 03, 2007; Accepted: December 26, 2007)

Abstract: The gene induced in response to Cd exposure in microalga Nannochloropsis oculata was examined in this investigation. In order to isolate this gene by using nested PCR, degenerated primers were designed on the basis of highly conserved regions of the amino acid sequences of various phytochelatin synthase. The size of the nested PCR amplification product from Nannochloropsis oculata by using two degenerated primers was measured to be 266 bp. The 266 bp DNA fragment was sequenced, and it might be induced by Cd exposure in microalga Nannochloropsis oculata.

Key words: Cadmium, DNA, Nannochloropsis oculata

PDF of full length paper is available with author (miyoung@sch.ac.kr)

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