Journal of Environmental Biology

Changes in activity of hepatic xenobiotic-metabolizing enzymes of tiger puffer (Takifugu rubripes) exposed to paralytic shellfish poisoning toxins

J.K. Jeon^*1, J.S. Lee¹, W.J. Shim², O. Aarakawa³, T. Takatani³, S. Honda⁴ and T. Noguchi⁵

¹Division of Marine Bioscience and Technology, Kangnung National University, and EMBRC, Gangneung, 210 702, Korea
²Eco-Environmental Research Laboratory, Korea Ocean Research and Development Institute, Ansan, 425 170, Korea
³Faculty of Fisheries, Nagasaki University, Nagasaki, 852 8521, Japan
⁴Graduate School of Science and Technology, Nagasaki University, Nagasaki, 852 8521, Japan
⁵Japan Frozen Foods Inspection Corporation, Minato, Tokyo, 105 0012, Japan

(Received: February 10, 2008; Revised received: April 04, 2008; Accepted: April 25, 2008)

Abstract: Attempts were made to examine the effect of paralytic shellfish poisoning toxins (PSP) on hepatic xenobiotic-metabolizing enzymes (XMEs) of tiger puffer (Takifugu rubripes). Two groups of nontoxic tiger fish were analyzed, and one group was fed with a PSP-containing diet (PSP group), and another with a PSP-free diet (control group). After 60 days of feeding, they were compared to each other, mainly in terms of the activity of XMEs. Both groups did not differ from each other significantly, in body weight gain, hepatosomatic index, and condition factor. Hepatic level of cytochrome P450 was lower in PSP group than control group. NADPH-cytochrome P450 reductase, NADH-cytochrome b5 reductase, and ethoxyresorufin-O-deethylase (EROD) exhibited a reduced activity in PSP group than control group. Statistical analysis found that the activity or concentration of those enzymes correlated with the hepatic level of PSP, with r2=0.497- 0.611.

Key words: Paralytic shellfish poisoning toxin (PSP), puffer fish, Takifugu rubripes, Xenobiotic metabolizing enzyme (XME)

PDF of full length paper is available with author (jkjeon@kangnung.ac.kr)

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