Journal of Environmental Biology

Cloning and expression analysis of a small HSP26 gene of  Pacific abalone
(Haliotis discus hannai)

Eun Mi Park^1,2, Young Ok Kim¹, Bo Hye Nam^*1, Hee Jeong Kong¹, Woo Jin Kim¹, Sang Jun Lee¹,
Young Ju Jee¹, In Soo Kong² and Tae Jin Choi^1,3

¹Biotechnology Research Institute, National Fisheries Research and Development Institute, 408-1 Sirang ri,
Gijang eup, Gijang gun, Busan 619-902, Republic of Korea
²Department of Biotechnology and Bioengineering, Pukyong National University, 599-1 Daeyeon dong, 
Nam gu, Busan 608-737, Republic of Korea
³Department of Microbiology, Pukyong National University, 599-1 Daeyeon dong, Nam gu, Busan 608-737, Republic of Korea

(Received: March 12, 2007; Revised received: February 18, 2008; Accepted: February 25, 2008)

Abstract: Heat shock proteins (HSPs) are evolutionally conserved from micro organism to mammals and play important roles in many biological processes including thermal tolerance. We isolated a homologue of small HSP26 (sHSP26) from a subtracted cDNA library of heat shock-treated abalone (Haliotis discus hannai). The abalone sHSP26 encompossed 793 nt, including a coding region of 501 nt. The deduced amino acid sequence of the abalone sHSP26 contained well conserved a-crystallin domain and showed overall identities of 27-31% with the other species’ sHSP proteins. The abalone sHSP26 transcript was induced by heat shock treatment, but not by cold shock treatment.

Key words: Pacific abalone, Heat shock protein, Small HSP26, Thermal stress

PDF of full length paper is available with author (bhnam@momaf.go.kr)

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