Journal of Environmental Biology

Heteropolymorphism of mitochondrial NADH dehydrogenase subunit 3 gene for the population analysis of chum salmon, Oncorhynchus keta

M. Yoon¹, Y.S. Choi¹, H.J. Jin¹, Y.C. Sohn¹, S.K. Lee² and D.H. Jin^*1

¹Faculty of Marine Bioscience and Technology, Kangnung National University, Gangneung 210-702, Korea
²College of Dentistry, Kangnung National University, Gangneung  - 210-702, Korea

(Received: May 08, 2007; Revised received: February 19, 2008; Accepted: Februart 23, 2008)

Abstract: Mitochondrial DNAs (mtDNAs) has been frequently used as genetic markers for the population genetic studies. In this study, we used chum salmon (Oncorhynchus keta) from Korea, Japan and America, and compared their mitochondrial NADH dehydrogenase subunit 3 (ND3) genes by DNA sequence analysis. Sequence variation was studied in the ND3 among total 11 individuals from three populations. The ND3 gene was amplified by PCR targeting parts of cytochrome oxidase III gene (COIII) and NADH dehydrogenase subunit 4L gene (ND4L). ND3 gene sequence, encoded 752 bps, presented some genetic variation in the chum salmon populations. The observed nucleotide variations inferred the distinct genetic differentiation of American salmons from Korean and Japanese chum salmons. Six sites of single nucleotide polymorphism (SNP) were explored in the ND3 locus. Denaturing gradient gel electrophoresis analysis also showed a clear heterogenous band in American salmons compared to Asian salmons.

Key words: Chum salmon, Mitochondrial NADH dehydrogenase gene, SNP

PDF of full length paper is available with author (dhjin@kangnung.ac.kr)

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