Journal of Environmental Biology

Detection of marine pathogenic bacterial Vibrio species by multiplex polymerase
chain reaction (PCR)

Mal Nam Kim* and Hyo Joo Bang

Department of Biology, Sangmyung University, Seoul - 110-743, Korea

(Received: February 03 ‘07; Revised received: October 26 ‘07; Re-revised received: November 05 ‘07; Accepted: November 27 ‘07)

Abstract: Polymerase chain reactions (PCR) were performed to accomplish quick and accurate detection of Vibrio species. The primers prepared with 16S-23S rDNA intergenic spacer (IGS) region exhibited an excellent species-specificity for Vibrio sp and detected Vibrio sp more successfully than the conventional culture method. Multiplex PCR was also fruitful not only for the identification of the 5 standard Vibrio sp simultaneously, but also for the detection of Vibrio sp in the samples collected from the natural environment.

Key words: Vibrio sp, Multiplex PCR, Species-specific primer

PDF of full length paper is available with author (mnkim@smu.ac.kr)

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