Journal of Environmental Biology

Phylogenetic analyses of Prorocentrum spp. and Alexandrium spp. isolated from Northern coast of Vietnam based on 18S rDNA sequence

Dang Diem Hong^*, Hoang Thi Minh Hien, Ngo Hoai Thu, Hoang Lan Anh and Quoc Hai Luyen

Institute of Biotechnology, Vietnamese Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay - 1000, Ha Noi, Vietnam

(Received: May 15, 2007; Revised received: February 22, 2008; Accepted: February 25, 2008)

Abstract: Some species of marine dinoflagellates belonging to genera Alexandrium and Prorocentrum have been responsible for paralytic shellfish poisoning (PSP) and diarrheic shellfish poisoning (DSP), respectively. Morphological and molecular studies of 4 species including Alexandrium sp. 5, Alexandrium sp. 16, Prorocentrum sp. 1 and Prorocentrum sp. 3 that were collected in Northern coast of Vietnam were presented for the first time. By morphologic observations, we identified Alexandrium sp. 5 and Alexandrium sp. 16 as Alexandrium minutum, Alexandrium affine, respectively; Prorocentrum sp. 1 and Prorocentrum sp. 3 as Prorocentrum mexicanum. Sequence data from the partial 18S riboxomal RNA genes have been used to generate a phylogenetic framework with database of GenBank. The obtained results of phylogenetic analyses of species of Prorocentrum spp. and Alexandrium spp. based on 18S rDNA sequences are similar to morphological observations. Thus, molecular tool would be helpful for the identification of dinoflagellate species and further taxonomic studies in Vietnam.

Key words: Alexandrium, Phylogenetic analysis, Prorocentrum, 18S rRNA gene

PDF of full length paper is available with author (ddhong60vn@yahoo.com)

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