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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP
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    Abstract - Issue Oct 2007, 28 (4)                                     Back


paper

Biophoton emission of MDCK cell with Hydrogen Peroxide and ?60 Hz AC magnetic field

 

?B.S. Cheun1, S.H. Yi*2, K.Y. Baik2, ?J.K. Lim2, J.S. Yoo3, H.W. Shin4 and?? K.S. Soh2

*seunghoyi@phya.snu.ac.kr

1Pacific Yale University Oriental Medicine, 333 East Colorado Boulevera Glendale, CA 91205, USA

2Laboratory of Biomedical Physics, School of Physics and Astronomy, Seoul National University, Seoul-151-747, South Korea

3Center Korea Institute of Marine Science and Technology Promotion, Seoul-137-941, South Korea

4Department of Marine Biotechnology, Soonchunhyang University, Asan 336-745, South Korea

(Received: May 16, 2006 ; Revised received: October 10, 2006 ; Accepted: November 17, 2006)

Abstract: We studied biophoton characteristics of Madin-Darby canine kidney (MDCK) cells under the influence of H2O2 by employing a photomultiplier tube (PMT) and a fluorescence microscope. H2O2 was used for producing reactive oxygen species (ROS) in the measurement. Images from a fluorescence microscope show an increase of photon intensity emitted from the sample due to H2O2. By using a PMT we measured quantitative change in biophoton emission with application of H2O2 to the MDCK cell culture, found that the increase of the biophoton is dependent upon the amount of H2O2. The agreement between the results of the PMT and the fluorescence microscope suggests the possibility of quantitative measurement of the influence of ROS on living tissue or cell. In addition we applied a 60 Hz AC magnetic field on the cells to investigate the change in reaction between MDCK cell and ROS. It showed that a decay of chemiluminescence intensity has taken a different path following exposure to the magnetic field. As a result, the PMT measurement might be considered as a useful tool for studying biochemical characteristics in relation to ROS.

Key words: ? Biophoton, MDCK, ROS, PMT, 60 Hz magnetic field

????????? PDF file of full length paper is available with author

 

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