Journal of Environmental Biology

Influence of cytokinine, a plant growth regulator (PGR) on the activity of some enzymes involved in metabolism, in the nymphs of Lipaphis  erysimi  (Kalt.)

 

S. K. Sohal, P. J. Rup  and G. K. Arora

Department of Zoology, Guru Nanak Dev University, Amritsar- 143 005, Amritsar, India

 

(Received: 26 July, 2004 ; Accepted: 8 February, 2005)

 

Abstract: Five enzymes were assessed for their activity under the influence of cytokinine (N⁶ Benzyladenine) (400ppm) in the nymphs (48-52 hr old) of mustard aphid, L. erysimi treated for 13, 25 and 37 hr. The activity of Glutathione S - transferases and Esterases increased with treatment as compared to control in the nymphs of the same age group. However the activity of Glutathione reductase was suppressed with treatment. NADH dehydrogenase activity and NADH oxidase activity in the nymphs was not affected much with the treatment. It was inferred that Glutathione S-transferases and Esterases might be involved in the degradation of cytokinine whereas, the other enzymes had no significant role to play in the metabolism of cytokinine.

 

Key words: Lipaphis erysimi, Cytokinine, Glutathione S-transferases, Esterases, NADH dehydrogenase, NADH oxidase, Glutathione reductase.

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