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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue May 2020, 41 (3)                                     Back


nstantaneous and historical temperature effects on a-pinene

Characterization of a novel antifungal protein from Acacia senegal seeds

                                                                                                                                             

D.S. Solanki1, S. Kumar1, K. Parihar1, A. Tak1, P. Gehlot1*, R. Pathak2 and S.K. Singh2 

1Department of Botany, Jai Narain Vyas University, Jodhpur-342 001, India

2Division of Plant Improvement and Pest Management, ICAR-Central Arid Zone Research Institute, Jodhpur-342 003, India

*Corresponding Author Email : drpg73@rediffmail.com

Paper received: 03.09.2019??????? ?????????????????????????????????????? Revised received: 23.11.2019? ??????????? ?????????????????????????????? Accepted: 26.11.2019

 

Abstract

Aim: The aim of the present study was to isolate antifungal protein from seeds of Acacia senegal in order to develop a new, effective and environmental friendly biofungicide.

Methodology: Antifungal protein from A. senegal seeds was extracted and purified through ammonium sulphate precipitation, dialysis, ion exchange and gel filtration chromatography. The novel antifungal was characterized employing SDS-PAGE, chitinase activity and antifungal efficacy. The purified protein was also characterized through MALDI-TOF MS/MS.          

Results: The yield of purified antifungal protein was estimated to be 0.96 mg 25 g-1 seeds and its molecular mass determined by SDS PAGE was 52.9 kDa. The purified protein exhibited antifungal activity against phytopathogenic fungi viz., Macrophomina phaseolina and Fusarium oxysporum also possessed chitinase activity. The purified protein was characterized through MALDI-TOF MS/MS and its spectra revealed 14 peptides with their specific amino acid sequences.??????? ???     

Interpretation: The antifungal protein isolated from A. senegal seeds has broad-spectrum antifungal activity with chitinase activity against pathogenic fungi that can be exploited for management of fungal disease as biopesticide to promote sustainable agriculture.? ?????   

Key words: Acacia senegal, Antifungal protein, Chitinase activity, MALDI TOF-Mass spectroscopy, Phytopathogenic fungi

 

 

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