aim of the present study was to isolate antifungal protein from seeds of
Acacia senegal in order to develop a new, effective and environmental
Methodology: Antifungal protein from A. senegal seeds was
extracted and purified through ammonium sulphate precipitation, dialysis, ion
exchange and gel filtration chromatography. The novel antifungal was
characterized employing SDS-PAGE, chitinase activity and antifungal efficacy.
The purified protein was also characterized through MALDI-TOF MS/MS.
Results: The yield of purified antifungal protein was
estimated to be 0.96 mg 25 g-1 seeds and its molecular mass
determined by SDS PAGE was 52.9 kDa. The purified protein exhibited
antifungal activity against phytopathogenic fungi viz., Macrophomina
phaseolina and Fusarium oxysporum also possessed chitinase
activity. The purified protein was characterized through MALDI-TOF MS/MS and
its spectra revealed 14 peptides with their specific amino acid sequences.???????
Interpretation: The antifungal protein isolated from A.
senegal seeds has broad-spectrum antifungal activity with chitinase activity
against pathogenic fungi that can be exploited for management of fungal
disease as biopesticide to promote sustainable agriculture.? ?????
Antifungal protein, Chitinase activity, MALDI TOF-Mass spectroscopy,