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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue May 2020, 41 (3)                                     Back

nstantaneous and historical temperature effects on a-pinene

Molecular characterization of phosphate solubilizing fungi Aspergillus niger and its correlation to sustainable agriculture


Y.S. Mazrou1,2*, A.H. Makhlouf3, E.R. Elbealy4, M.A. Salem5, M.A. Farid6, M.F. Awad7, 8, M.M. Hassan7,9 and M. Ismail9 

1Department of Business Administration Department, Community College, King Khaled University, Abha, 62529, Saudi Arabia

2Department of Agric. Economics, Faculty of Agriculture, Tanta University, Tanta, 3111, Egypt

3Department of Botany, Faculty of Agriculture, Menoufia University,? Menoufia, 32514, Egypt

4Department of Biology, Faculty of Science, King Khalid University, Abha, 61413, Saudi Arabia

5Department of Chemistry, Faculty of Science and Art, King Khalid University, Asir, 61421, Saudi Arabia

6Department of Biology, Faculty of Science, Taif University,? Taif, 21944, Saudi Arabia

7Department of Genetics , Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh,33516, Egypt

8Department of Botany and Microbiology, Faculty of Science, Al-azhar University, Assuit, 71511, Egypt

9Department of Genetics, Faculty of Agriculture, Menoufia University, Menoufia, 32514, Egypt

*Corresponding Author Email :

Paper received: 14.10.2019??????? ?????????????????????????????????????? Revised received: 15.01.2020????????????? ???????????????????????????????????? Accepted: 28.02.2020



Aim: The present study aimed to investigate the phosphate solubilization potential of agriculturally important fungi, i.e., Aspergillus sp. isolated from the rhizosphere of healthy plants in Abha city, Saudi Arabia.

Methodology: Sixteen Aspergillus sp. isolated and tested for phosphate solubilization potential were identified by 5.8S-ITS region sequencing and characterized by 11 ISSR-PCR markers. Finally, the highest phosphate solubilization potential isolates were used in field experiments on cucumber and tomato plants.          

Results: All Aspergillus niger isolates showed 96?100% similarity to A. niger strains available at GenBank database, Isolate ASAB-5 was most efficient at solubilizing phosphate on Pikovskaya?s medium, with a solubilization index of 2.67, and 235.22 mg l-1 of solubilized phosphate. ISSR-PCR markers revealed is total 142 bands in all isolates, with about 32.3% showing monomorphism and 67.6% polymorphism. Based on genetic similarity and intraspecies variability, the Aspergillus isolates were grouped into two different clusters with about 67.9% genetic similarity. The results of field experiments showed no significant difference between seeds treated with culture filtrate or conidial suspension of ASAB-5; however, both differed remarkably from untreated seeds.??      

Interpretation: The current study confirms the existence of several useful phosphate solubilizing fungi in plants, which may serve as potential biological fertilizers. They are safer than chemical fertilizers and increase the bioavailability of soil phosphates for plants.??    

Key words: Aspergillus niger, ISSR markers, Phosphate solubilization, 5.8S-ITS sequencing 



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