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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue July 2011, 32 (4)                                     Back

nstantaneous and historical temperature effects on a-pinene

Freeze survival of the cyanobacteria

Microcoleus chthonoplastes without cryoprotector


Author Details


Erick J. N??ez-V?zquez

(Corresponding author)

Centro de Investigaciones Biol?gicas del Noroeste S. C. (CIBNOR), Apdo. Post. 128,

La Paz, B. C. S. 23000, M?xico


Dariel Tovar-Ram?rez

Centro Investigaciones Biol?gicas del Noroeste (CIBNOR), Apdo. Post. 128, La Paz, B.C.S. 23000, M?xico

Alejandra Heredia-Tapia

Investigaci?n para la Conservaci?n y el Desarrollo A. C. (INCODE), Andador 2, No. 245. Col.

Banobras, La Paz, B. C. S. 23080, M?xico

Jos? Luis Ochoa

Centro Investigaciones Biol?gicas del Noroeste (CIBNOR), Apdo. Post. 128, La Paz,

B.C.S. 23000, M?xico



Publication Data

Paper received:

31 August 2010


Revised received:

2 July 2010



23 September 2010



A Microcoleus chthonoplastes strain SC7B9002-1 isolated from microbial mats in tidal channels from San Carlos, Baja California Sur, Mexico was subjected to short- (15 days) and long-term (2 years) conservation assays in liquid nitrogen (?196oC) using cryoprotective agents, such as 5% DMSO, 20% PVP-40, and 20% glycerol. Survival rate, chlorophyll a, protein, and nucleic acids content were observed in each case. Interesting growth and a significant increase in protein content was observed when no cryoprotectant was used during liquid nitrogen immersion. In the absence of a cryoprotectant, M. chthonoplastes lost their typical shape resembled spheroplasts, and recovery cultivation times after freezing were 5 and 25 days (short and long-term, respectively). Recovery from long-term preservation with 5% DMSO took 15 days. PVP and glycerol did not allow recovery of viable cells. The survival of M. chthonoplastes to freezing without cryoprotectant and the adaptive mechanisms that allow surviving under freezing conditions are discussed.


Key words

Freezing-survival, Cryopreservation, Cyanobacteria, Microcoleus chthonoplastes


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