JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

About Journal
    Home
    Editor in Chief
    Editorial Board
    Reviewer Panel
    Publication Policies
    Guidelines for Editors
    Guidelines for Reviewers
    Abstracting and Indexing
    Subscription and Payments
    Contact Journal
 
Read Journal
    Current Issue
    Journal Archives
 
For Authors
    Guidelines for Authors
    Terms and Conditions
    Fees and Payments
    Track Paper Status
 
Announcements
    JEB Awards
 

Google Search the Journal web-site:


    Abstract - Issue July 2011, 32 (4)                                     Back


nstantaneous and historical temperature effects on a-pinene

Freeze survival of the cyanobacteria

Microcoleus chthonoplastes without cryoprotector

 

Author Details

 

Erick J. N??ez-V?zquez

(Corresponding author)

Centro de Investigaciones Biol?gicas del Noroeste S. C. (CIBNOR), Apdo. Post. 128,

La Paz, B. C. S. 23000, M?xico

e-mail: enunez04@cibnor.mx, erick@incodeac.org

Dariel Tovar-Ram?rez

Centro Investigaciones Biol?gicas del Noroeste (CIBNOR), Apdo. Post. 128, La Paz, B.C.S. 23000, M?xico

Alejandra Heredia-Tapia

Investigaci?n para la Conservaci?n y el Desarrollo A. C. (INCODE), Andador 2, No. 245. Col.

Banobras, La Paz, B. C. S. 23080, M?xico

Jos? Luis Ochoa

Centro Investigaciones Biol?gicas del Noroeste (CIBNOR), Apdo. Post. 128, La Paz,

B.C.S. 23000, M?xico

 

 

Publication Data

Paper received:

31 August 2010

 

Revised received:

2 July 2010

 

Accepted:

23 September 2010

 

Abstract

A Microcoleus chthonoplastes strain SC7B9002-1 isolated from microbial mats in tidal channels from San Carlos, Baja California Sur, Mexico was subjected to short- (15 days) and long-term (2 years) conservation assays in liquid nitrogen (?196oC) using cryoprotective agents, such as 5% DMSO, 20% PVP-40, and 20% glycerol. Survival rate, chlorophyll a, protein, and nucleic acids content were observed in each case. Interesting growth and a significant increase in protein content was observed when no cryoprotectant was used during liquid nitrogen immersion. In the absence of a cryoprotectant, M. chthonoplastes lost their typical shape resembled spheroplasts, and recovery cultivation times after freezing were 5 and 25 days (short and long-term, respectively). Recovery from long-term preservation with 5% DMSO took 15 days. PVP and glycerol did not allow recovery of viable cells. The survival of M. chthonoplastes to freezing without cryoprotectant and the adaptive mechanisms that allow surviving under freezing conditions are discussed.

 

Key words

Freezing-survival, Cryopreservation, Cyanobacteria, Microcoleus chthonoplastes

 

Copyright ? 2011 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).