nstantaneous and historical temperature effects on a-pinene
Freeze survival of the cyanobacteria
Centrode InvestigacionesBiol?gicasdelNoroeste S.
C. (CIBNOR), Apdo. Post. 128,
B. C. S. 23000, M?xico
CentroInvestigacionesBiol?gicasdel Noroeste (CIBNOR), Apdo. Post. 128, La Paz, B.C.S. 23000, M?xico
Investigaci?n para la Conservaci?n y el Desarrollo A.
C. (INCODE), Andador 2, No. 245. Col.
Banobras, La Paz, B. C. S. 23080, M?xico
CentroInvestigacionesBiol?gicasdel Noroeste (CIBNOR), Apdo. Post. 128, La Paz,
B.C.S. 23000, M?xico
23 September 2010
AMicrocoleuschthonoplastes strain SC7B9002-1 isolated from
microbial mats in tidal channels from San Carlos, Baja California Sur, Mexico was subjected to short- (15 days) and
long-term (2 years) conservation assays in liquid nitrogen (?196oC)
using cryoprotective agents, such as 5% DMSO, 20%
PVP-40, and 20% glycerol. Survival rate, chlorophyll a, protein, and nucleic
acids content were observed in each case. Interesting growth and a
significant increase in protein content was observed when no cryoprotectant was used during liquid nitrogen immersion.
In the absence of a cryoprotectant, M. chthonoplastes lost their typical shape resembled spheroplasts, and recovery cultivation times after
freezing were 5 and 25 days (short and long-term, respectively). Recovery
from long-term preservation with 5% DMSO took 15 days. PVP and glycerol did
not allow recovery of viable cells. The survival of M. chthonoplastes
to freezing without cryoprotectant and the adaptive
mechanisms that allow surviving under freezing conditions are discussed.
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