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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Sep 2024, 45 (5)                                     Back


nstantaneous and historical temperature effects on a-pinene

Short-term dietary supplementation of β-glucan and chitosan modulates the immune response in freshwater prawn, Macrobrachium rosenbergii (De Man, 1879)

 

S. Sahoo1, M.R. Badhe1, A. Paul1, P.K. Sahoo1, K.C. Das1, D. Panda1, B.R. Pillai1, S. Baliarsingh2, B.B. Patnaik2 and J. Mohanty1*

1ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar–751 002, India

2P.G. Department of Biosciences and Biotechnology, Fakir Mohan University, Balasore–756 089, India

 

Received: 26 February 2024                   Revised: 15 May 2024                   Accepted: 05 July 2024

*Corresponding Author Email : jmohantycifa@gmail.com                 *ORCiD: https://orcid.org/0000-0002-5748-7054

 

 

 

Abstract

 

Aim: The present study evaluates the immunomodulatory potentials of β-glucan and chitosan administered orally for 15 days to the freshwater prawn, Macrobrachium rosenbergii juveniles.

Methodology: Two diets were formulated supplementing 1.5 g kg-1 and 20 g kg-1 of β-glucan (diet G) and chitosan (diet C), respectively, with a control diet (diet B). The prawns were fed twice daily at 5% biomass. At the end of the feeding trials, mRNA expression profiles of immune-related genes lipopolysaccharide- and β-1,3-glucan binding protein (LGBP) and chitinase 3 were analyzed in qPCR. Corresponding LGBP protein and chitinases/CBP (chitinase 1, chitinase 1C and obstructor E, a chitin-binding protein) protein levels were also estimated by ELISA.

Results: Supplementation of the immunostimulants (diets G and C) significantly enhanced the mRNA expression of both LGBP and chitinase 3 in the hepatopancreas. The protein expression of LGBP and chitinases/CBP was also significantly increased in the serum and hepatopancreas samples of both treatment groups.

Interpretation: The short-term supplementation of β-glucan and chitosan in diets demonstrated beneficial effects in M. rosenbergii by enhancing the expression of important immune molecules like LGBP and chitinases, indicating their potential applications during the period of biotic stress.

Key words: β-glucan, Chitinase, Hepatopancreas, Immunostimulants, LGBP, Macrobrachium rosenbergii

 

 

 

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