Abstract
Aim:
Doubled
haploid technology was utilized to accelerate the development of maize lines
with traits suitable for high planting density, enabling the identification
of potential parental lines for hybrid development under these conditions.
Methodology:
Two
F1 populations (PML-119 x PML-185 and PML-95 x PML-172) were used to generate
doubled haploid lines, confirmed using polymorphic markers phi115 and umc1887.
These lines were phenotypically evaluated under high density (60 cm x 15 cm)
and normal density (60 cm x 20 cm) conditions.
Results:
Using
doubled haploid technology, 67 lines were developed from two source
populations with traits such as narrow leaf angle, moderate plant height, and
sparse tassel for high planting density stress tolerance. Molecular marker
screening confirmed the homozygosity of these lines. Agronomic evaluation
under narrow plant-to-plant spacing (15 cm) identified eight potential donor
lines amenable to high planting density stress, ready for use as parents in
breeding high-density tolerant hybrids.
Interpretation:
Potential
donor doubled haploid lines viz. DH2-4, DH2-8, DH2-9,
DH2-11, DH2-14, DH2-15 derived from
Population-3 X CIM2GTAIL P2 and DH2-4, DH2-23 derived
from Population-9 X CIM2GTAIL P2 were identified for utilization as putative
parents of hybrids suitable for planting under high planting density
conditions.
Key
words: Doubled
haploid, High plant density, Maize, Plant architecture, Yield
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