JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

About Journal
    Obituary: Dr. R. C. Dalela
    Editorial Board
    Reviewer Panel
    Publication Policies
    Guidelines for Editors
    Guidelines for Reviewers
    Abstracting and Indexing
    Subscription and Payments
    Contact Journal
    About Triveni Enterprises
Read Journal
    Current Issue
    Journal Archives
For Authors
    Guidelines for Authors
    Terms and Conditions
    Fees and Payments
    Track Paper Status

Google Search the Journal web-site:

    Abstract - Issue Jan 2023, 44 (1)                                     Back

nstantaneous and historical temperature effects on a-pinene

Morpho-genetic variability of Rhizoctonia solani population causing sheath blight disease in rice (Oryza sativa L.)


S. Chaudhary1, S. Sagar1, M. Lal2, A. Tomar3, J. Kumar1, V. Kumar4 and M. Kumar1*   


1Department of Agricultural Biotechnology, College of Agriculture, Sardar Vallabhbhai Patel University of Agriculture and Technology,

Meerut-250110, India

2Division of Plant Protection, ICAR-Central Potato Research Institute (Regional Station), Meerut-250110, India

3Department of Recombination Techniques, College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology,

Meerut-250 110, India

4ICAR-National Institute of Biotic Stress Management, Raipur-493 225, India

*Corresponding Author Email :                      *ORCiD:


Received: 03.04.2021                                                                                           Revised: 18.06.2021                                                                Accepted: 25.06.2022




Aim: The present study aims to investigate the morpho-pathological and molecular variability among the R. solani isolates from different geographical regions of India.

Methodology: R. solani isolates were collected from sheath blight infected rice plants from diverse regions of Western Uttar Pradesh and Uttarakhand. Morphological variability among the isolates was studied in-vitro on PDA medium. The anastomosis grouping was determined by hyphal fusion reactions paired with tester strain and confirmed by AG-specific-PCR assay. Pathogenicity assay was conducted in-vivo on four rice varieties using artificial inoculation method under greenhouse conditions. Evolutionary relationship among the isolates was determined using rDNA-ITS-PCR with ITS1 & ITS4 primers. Further, genetic variability among the isolates was assessed using ISSR primers.

Results: A total of 21 isolates of R. solani were recovered and based on morphological and sclerotial features, significant variation was observed among the isolates. All the isolates belonged to AG1-IA group which was confirmed by AG-specific PCR assay. Based on pathogenicity, eight isolates were found highly virulent, eight were moderately virulent and six were less virulent. ISSR markers showed high level of polymorphism and grouped all isolates into three major clusters showing partial correlation with geographical origin of the isolates. Sequence variations in ITS region were observed in the form of insertions or deletions of the nucleotide when rDNA-ITS sequences were compared that led to the interspecies diversity and diversity was observed in clad-specific manner. The phylogenetic tree separated the isolates into ten distinct clades showing high level of genetic diversity.

Interpretation: The results of the present study may be helpful for the phylogenetic classification of complex R. solani species and provide information on the genetic divergence and occurrence of pathogenic races. This information will be helpful for the development of effective disease management strategies based on the molecular breeding and other approaches.


Key words: Anastomosis, Genetic diversity, ISSR, Pathogenicity, R. solani, Sheath blight




Copyright © 2023 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).