JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

About Journal
    Home
    Obituary: Dr. R. C. Dalela
    Editorial Board
    Reviewer Panel
    Publication Policies
    Guidelines for Editors
    Guidelines for Reviewers
    Abstracting and Indexing
    Subscription and Payments
    Contact Journal
    About Triveni Enterprises
 
Read Journal
    Current Issue
    Journal Archives
 
For Authors
    Guidelines for Authors
    Terms and Conditions
    Fees and Payments
    Track Paper Status
 

Google Search the Journal web-site:


    Abstract - Issue Jul 2022, 43 (4)                                     Back


nstantaneous and historical temperature effects on a-pinene

Revealing Berberis aristata potential as an anti-viral agent to combat Paramyxoviridae infection

 

Y.S. Shreenivasan, S. Keerthana, A. Praveena* and P. Dhasarathan

Department of Biotechnology, Prathyusha Engineering College, Chennai-602 025, India

*Corresponding Author Email : praveena_bioinfo@yahoo.com

 

Received: 06.04.2021                                                                                                    Revised: 10.08.2021                                                                         Accepted: 03.01.2022

 

 

Abstract

Aim: To study the anti-viral activity of extracts of Berberis aristata and to scrutinize the novel lead compound present in it probably to combat Paramoxyviridae infection.

Methodology: The phytochemicals present in the barks of Berberis aristata were extracted and screened by GC-MS analysis. Haemagglutination inhibition and cytotoxicity assay was performed to determine the anti-viral property, and the novel lead compound was selected using in-silico methods.

Results: Haemagglutination assay provided anti-viral activity of the extract at 1/16 dilution in 4 HA viral concentration. At this concentration, the viability on Vero cell lines was precisely 92.8%. The GC-MS analysis enabled in identifying six molecules present in the extract. Among the six compounds present in the extract, five moieties exhibited drug likeliness property when passed through the Lipinski’s drug filter. QSAR predictions using T.E.S.T projected 3 compounds to be developmental non-toxicant with the predicted values of 0.12, 0.32 and 0.42 respectively. On performing docking studies with the predicted nontoxic moieties using iGEMDOCK, with the Sialic acid complexes host receptor, the highest binding energy was -213 kcal mol-1 for alpha-d-mannofuranoside, 1-o-decyl-, respectively.

Interpretation: These findings enabled in understanding the anti-viral potency of bark extracts of B. aristata at 62.5mg ml-1 promoting high cellular viability of uninfected cells of host cell with low toxic effects. Probing molecularly, the in-silico analysis helped to predict alpha-d-mannofuranoside, 1-o-decyl as the possible lead molecule supporting its therapeutic efficacy as an anti-viral drug compound in future.

Key words: Acid dye method, Developmental non-toxicant, Haemagglutination assay, Lipinski’s drug filter

 

 

 

Copyright © 2022 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).