Expression
profiling of flavonoid biosynthesis genes in association with accumulation of
flavonoid compounds in rice under water deficit stress
K.
Jayaraman1,2, A.M. Sevanthi1, V.K. Raman1
and P.K. Mandal1*
1ICAR - National
Institute for Plant Biotechnology, New Delhi-110 012, India
2Department of
Botany, Bharathidasan University, Tiruchirappalli-637 024, India
*Corresponding
Author Email : pranabkumarmandal@gmail.com
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Abstract
Aim:
To investigate whether the phenolic and flavonoid contents have any direct
relation with the water deficit stress tolerance in rice. If so, then, which
genes in the flavonoid biosynthesis pathway are involved in the tolerance mechanism
was explored by comparing a well-known water deficit tolerant cv. Nagina 22
(N22) as experiment material along with a sensitive cv. Pusa Sugandh 2 (PS2).
Methodology: Water deficit stress was imposed on N22 (drought
tolerant) and PS2 (drought sensitive) cultivars at the seedling stage by
withholding water for 7days. Further, the expression level of flavonoid
biosynthesis genes (OsCHS, OsCHI, OsF3H, OsF3’H, OsDFR, and OsANS) and
the accumulation of total contents of phenolics and flavonoids in rice leaves
of N22 and PS2 under water deficit stress were also investigated.
Results:
Quantitative RT-PCR analysis revealed enhanced expression of six major genes
of the flavonoid biosynthesis pathway, namely OsCHS, OsCHI, OsF3H, OsF3’H,
OsDFR, and OsANS in the drought tolerant cv. N22 compared to the
drought sensitive cv. PS2 under water deficit stress. Accumulation of total
phenolics and flavonoids compounds increased during water deficit stress in
both the genotypes, but, it was significantly higher in N22 as compared to
PS2.
Interpretation: Our findings conclude that enhanced
drought tolerance is positively correlated with an enhanced transcription of
flavonoid biosynthesis genes and higher flavonoid content, suggesting that
there could be differences in the genetic control of flavonoid metabolism in
rice for drought stress response that, the enhanced expression and
accumulation of flavonoids is promising strategy for rice improvement.
Key words: Antioxidant capability, Flavonoids, Nagina22, Rice,
Water deficit tolerance
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