JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

About Journal
    Obituary: Dr. R. C. Dalela
    Editorial Board
    Reviewer Panel
    Publication Policies
    Guidelines for Editors
    Guidelines for Reviewers
    Abstracting and Indexing
    Subscription and Payments
    Contact Journal
    About Triveni Enterprises
Read Journal
    Current Issue
    Journal Archives
For Authors
    Guidelines for Authors
    Terms and Conditions
    Fees and Payments
    Track Paper Status

Google Search the Journal web-site:

    Abstract - Issue Jul 2022, 43 (4)                                     Back

nstantaneous and historical temperature effects on a-pinene

Production of multi enzyme preparation by Bacillus subtilis

using mosambi peel as substrate


B. Singh1,2, N. Garg2*, P. Mathur1, S. Vaish2 and S. Kumar2

1Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow-226 028, India

2Division of Post Harvest Management, ICAR-CISH, Lucknow-226 101, India

*Corresponding Author Email :


Received: 14.09.2021                                                                                                Revised: 03.01.2022                                                                                     Accepted: 31.03.2022




Aim: To determine feasibility of using mosambi peel as substrate for multi enzyme production through microbial intervention.

Methodology: Thirty-three bacterial isolates were isolated from biodegradable organic substrates and investigated in-vitro their biodegradable activities viz. pectinolytic, cellulolytic and amylolytic. The best performing bacterial isolate, exhibiting aforesaid activities were selected and identified using 16S rRNA technique. The factors, that influenced the fermentation, viz. temperature, pH and incubation period of bacterial culture were optimized for maximum production of multi enzymes viz. pectinase, cellulase and amylase using mosambi peel as substrate under solid-state fermentation conditions. Molecular weights of different enzymes present in multi-enzyme preparation were determined by SDS PAGE. The juice extraction efficiency of crude multi enzyme preparation was compared with that of pure commercial enzyme.

Results: Out of thirty-three bacterial isolates, after primary and secondary screening, the best performing bacterial isolate was identified as Bacillus subtilis strain NG 105 (Genbank accession number MN493055). Higher enzyme activities were observed at pH 7.0, incubation temperature of 35°C and incubation period of 10 days for pectinase, cellulase and amylase using mosambi peel as substrate. The purified enzymes characterized by SDS-PAGE, revealed to have molecular mass of 65 kDa for pectinase, 50 kDa for cellulase and 55 kDa for amylase. Bagasse fibre was found to be the most suited matrix for immobilization. The juice extraction efficiency of partially purified crude multienzyme preparation was 88% of commercial pectinase.

Interpretation: Mosambi peel is a suitable substrate for multienzyme production using Bacillus subtilis under solid-state fermentation condition.

Key words: Amylase, Bacillus subtilis, Cellulase, Immobilization, Mosambi peel, Pectinase




Copyright © 2022 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).