JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

About Journal
    Obituary: Dr. R. C. Dalela
    Editorial Board
    Reviewer Panel
    Publication Policies
    Guidelines for Editors
    Guidelines for Reviewers
    Abstracting and Indexing
    Subscription and Payments
    Contact Journal
    About Triveni Enterprises
Read Journal
    Current Issue
    Journal Archives
For Authors
    Guidelines for Authors
    Terms and Conditions
    Fees and Payments
    Track Paper Status

Google Search the Journal web-site:

    Abstract - Issue Nov 2021, 42 (6)                                     Back

nstantaneous and historical temperature effects on a-pinene

Bactericidal effect of Neem (Azadirachta indica) leaf extract on Helicobacter pylori


A. Saxena1, P. Arivaradarajan1, A.K. Mukhopadhyay2 and S.P. Nandi3*

1Amity Institute of Biotechnology, Amity University, Noida-201 313, India

2Bacteriology Division, ICMR-National Institute of Cholera and Enteric Diseases, Kolkata-700 010, India

3Centre for Cellular and Molecular Biotechnology, Amity Institute of Biotechnology, Amity University, Noida-201 313, India

*Corresponding Author Email :


Received: 15.07.2021                                                                    Revised: 02.09.2021                                            Accepted: 27.09.2021




Aim: The aim of the present study was to investigate the antibacterial effect of ethanolic extract of neem (Azadirachta indica) leaf against Gram-negative, gastric pathogen, Helicobacter pylori (H. pylori).

Methodology: Extracts of neem leaf were prepared in different solventslike hexane, dichloromethane, chloroform, ethyl acetate, acetone and ethanol. Antibacterial activity was estimated in terms of zone of inhibition by performing Agar cup diffusion assay. Depending on the diameter of zone of inhibition, ethyl acetate, acetone and ethanol extract of neem leaves were selected for Thin Layer Chromatography. The presence of photochemicals were detected using iodine fumigation. Elution Assay was done to detect the bioactive components of the ethanol extract.

Results: Out of sixsolvents used, ethanol extract of neem leavesshowed the maximum zone of inhibition against H. pylori. TLC separation of ethyl acetate, acetone and ethanol extract of plant products showed dark brown bands of phytochemicals on silica-gel G 60 plates. The contact bioautography assay showed a zone of 15 mm. Elution assay and agar cup bioassay was performed against H. pylori and the loading spot showed a zone of 11 mm.

Interpretation: The findings of the present study revealed the anti-bacterial potency of ethanolic extracts of neem (Azadirachta indica) leaf against Gram-negative gastric pathogen H. pylori. The ethanolic extract of neem leaf can be used as an effective natural remedy in combating H. pylori infection.       

Key words: Agar cup diffusion assay, Azadirachta indica, Helicobacter pylori, Phytochemicals




Copyright © 2021 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).