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Abstract - Issue Sep 2021, 42 (5) Back
nstantaneous and historical temperature effects on a-pinene
Effect of culture
media, auxins and genotypes on plantlet regeneration from oil palm (Elaeis
guineensis Jacq.) zygotic embryos through somatic embryogenesis
P.N. Kumar2, S.R.K. Motukuri1*, D. Ramajayam3,
B. Susanthi4 and H.S. Prasanna5
Education Foundation, Deemed to be University, Vaddeswaram - 522 502, India
of Floricultural Research, Pune - 411 005, India
Research Centre for Banana, Tiruchirapally - 620 102, India
Institute of Oil Palm Research, Pedavegi-534 450, India
Horticulture, University of Horticultural Sciences, Bagalkot-587 104, India
*Corresponding Author Email : email@example.com
24.03.2021 Accepted: 04.05.2021
This study evaluated efficient culture media for the regeneration of elite
material through somatic embryogenesis from oil palm zygotic embryos.
Methodology: For callus induction, zygotic embryos of four elite
genotypes (G1-264T, G2-238DX17P, G3-37DX17P and G4-237T) were cultured on
three basal media (Y3, MS and N6) with different auxin 2 mg l-1
(Picloram, 2,4-D and Dicamba) combinations. Subculture was made every month
for three passages. It evaluated various callus characters. The embryogenic
calli from callus induction media were transferred to the embryo maturation
medium and subcultured until the polyembryoids formed. For shoot and root
formation, somatic embryo clumps were transferred into regeneration media.
In-vitro plantlets with well-grown roots were hardened in pots for six weeks
and assessed clonal fidelity using polymorphic SSR primers.
Among the treatments, calli from N6+2,4-D, Y3+2,4-D and N6+Picloram showed
the highest embryogenic callus potential. G4-237T induced more embryogenic
calli (32.982) among genotypes, which was on par with G1-264T (24.196).
Embryogenic calli grown on N6 media with Dicamba showed the highest
proliferation rate (1.141). After 60 days of culture on regeneration media,
the highest number of plantlets per somatic embryogenic clump was obtained
from G1-264T on N6 media supplemented with Dicamba.
Interpretation: Culture media salt concentration showed
a significant difference among media by causing perturbations of auxin flow
during somatic embryogenesis affecting callus induction, proliferation and
plantlet regeneration. This may be useful for standardizing the
genotype-specific regeneration media in oil palm.
Auxin, Genotype, Oil palm, Somatic embryogenesis, Zygotic embryo
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