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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Sep 2021, 42 (5)                                     Back

nstantaneous and historical temperature effects on a-pinene

A medicinal plant Cudrania tricuspidata ameliorates allergic inflammation in mouse


Y.S. Kim1, A.R. Ryu2, C.R.Yoon2, J.W. Choi2, J.R. Huh3 and M.Y. Lee1,2* 

1Department of Medical Science, College of Medical Sciences, Soonchunhyang University, Asan, 22 Soonchunhyang-ro, 31538, Republic of Korea

2Department of Medical Biotechnology, College of Medical Sciences, Soonchunhyang University, Asan, 22 Soonchunhyang-ro, 31538, Republic of Korea

3College of Engineering, Konkuk University, Seoul, 120 Neungdong-ro, 05029, Republic of Korea

*Corresponding Author Email :


Received: 27.10.2020                                                                           Revised: 15.03.2021                                                          Accepted: 08.06.2021




Aim: This study aims at investigating the effect of ethanol extract of Cudrania tricuspidata twigs on in vitro and in vivo allergic inflammation.

Methodology: The ethanol extract of twigs from Cudrania tricuspidata (CTE) was prepared. The effects of CTE on the lipopolysaccharide (LPS)-induced production of nitric oxide (NO) in Raw 264.7 cells were examined. 2,4-Dinitrochlorobenzene (DNCB)-induced allergic inflammation model of mouse was constructed for in vivo study. The anti-oxidative, anti-inflammatory and anti-allergic effects of CTE were examined through DPPH (2,2-diphenyl-1-picrylhydrazyl) assay, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, enzyme-linked immunosorbent assay (ELISA) and Western blot analysis.

Results: The LPS-induced NO level was significantly reduced by Cudrania tricuspidata application in Raw 264.7 cells. LPS-induced inducible nitric oxide synthase (iNOS) expression was also reduced by CTE, showing CTE’s anti-inflammatory activity. Moreover, CTE reduced DNCB-induced IgE level in the mouse serum. The suppressive effect on the allergic inflammation stimulated by DNCB in the mouse model was investigated in terms of IL-4, IFN-γ, TNF-α level and PAR-2 expression.      

Interpretation: The ethanol extract of Cudrania tricuspidata twigs can be developed as a potent therapeutics for allergic inflammation.

Key words: Allergic inflammation, Cudrania tricuspidata, Cytokine, IgE, PAR-2




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