JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

About Journal
    Home
    Editor in Chief
    Editorial Board
    Reviewer Panel
    Publication Policies
    Guidelines for Editors
    Guidelines for Reviewers
    Abstracting and Indexing
    Subscription and Payments
    Contact Journal
 
Read Journal
    Current Issue
    Journal Archives
 
For Authors
    Guidelines for Authors
    Terms and Conditions
    Fees and Payments
    Track Paper Status
 
Announcements
    JEB Awards
 

Google Search the Journal web-site:


    Abstract - Issue Sep 2021, 42 (5)                                     Back


nstantaneous and historical temperature effects on a-pinene

A medicinal plant Cudrania tricuspidata ameliorates allergic inflammation in mouse

 

Y.S. Kim1, A.R. Ryu2, C.R.Yoon2, J.W. Choi2, J.R. Huh3 and M.Y. Lee1,2* 

1Department of Medical Science, College of Medical Sciences, Soonchunhyang University, Asan, 22 Soonchunhyang-ro, 31538, Republic of Korea

2Department of Medical Biotechnology, College of Medical Sciences, Soonchunhyang University, Asan, 22 Soonchunhyang-ro, 31538, Republic of Korea

3College of Engineering, Konkuk University, Seoul, 120 Neungdong-ro, 05029, Republic of Korea

*Corresponding Author Email : miyoung@sch.ac.kr

 

Received: 27.10.2020                                                                           Revised: 15.03.2021                                                          Accepted: 08.06.2021

 

 

Abstract

Aim: This study aims at investigating the effect of ethanol extract of Cudrania tricuspidata twigs on in vitro and in vivo allergic inflammation.

Methodology: The ethanol extract of twigs from Cudrania tricuspidata (CTE) was prepared. The effects of CTE on the lipopolysaccharide (LPS)-induced production of nitric oxide (NO) in Raw 264.7 cells were examined. 2,4-Dinitrochlorobenzene (DNCB)-induced allergic inflammation model of mouse was constructed for in vivo study. The anti-oxidative, anti-inflammatory and anti-allergic effects of CTE were examined through DPPH (2,2-diphenyl-1-picrylhydrazyl) assay, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, enzyme-linked immunosorbent assay (ELISA) and Western blot analysis.

Results: The LPS-induced NO level was significantly reduced by Cudrania tricuspidata application in Raw 264.7 cells. LPS-induced inducible nitric oxide synthase (iNOS) expression was also reduced by CTE, showing CTE’s anti-inflammatory activity. Moreover, CTE reduced DNCB-induced IgE level in the mouse serum. The suppressive effect on the allergic inflammation stimulated by DNCB in the mouse model was investigated in terms of IL-4, IFN-γ, TNF-α level and PAR-2 expression.      

Interpretation: The ethanol extract of Cudrania tricuspidata twigs can be developed as a potent therapeutics for allergic inflammation.

Key words: Allergic inflammation, Cudrania tricuspidata, Cytokine, IgE, PAR-2

 

 

 

Copyright © 2021 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).