Morpho-molecular analyses for taxonomic characterization of nine predominant Penicillium
species present in the soil of different parts of India.
Methodology: Fifteen Penicillium isolates were isolated
from the soil samples collected from the experimental field of ICAR-Indian
Agricultural Research Institute (IARI), New Delhi. Another twenty-six
isolates were procured from Indian Type Culture Collection (ITCC), Division
of Plant Pathology, ICAR-IARI, New Delhi which were isolated from the soil of
different parts of India. Total 41 isolates were characterized following
distinct macroscopic (colony texture, colony colour exudate production;
soluble pigmentation; reverse coloration and mycelial growth) and microscopic
observations (type of penicillus; shape of phialides; conidial shape,
size and pigmentation). Molecular characterization was done using partial
β-tubulin gene sequence which is considered an excellent marker in
differentiating Penicillium species.
The morphological data in species description perfectly matched with
molecular data generated using β-tubulin gene marker and nine different
species viz., P. aethiopicum, P. chrysogenum, P. crustosum,
P. janthinellum, P. mononematosum, P. oxalicum, P.
polonicum, P. singorense and Talaromyces pinophilus (Syn. P.
pinophilum) were confirmed.
Interpretation: Above nine Penicillium species
are found predominantly in the soil collected from different parts of India.
The β-tubulin gene can be considered as an excellent marker to differentiate Penicillium
species as confirmed from this study. The combined morpho- molecular
analyses can be further utilized to delineate morphologically similar Penicillium
species and also helpful to establish new species of Penicillium.
β-tubulin gene, Morpho-molecular approach, Penicillium species