Abstract
Aim:
This study aimed to search for novel cellulolytic isolates with high
cellulase titre for the production of fuels and chemicals.
Methodology: The yeast isolate YES5 isolated from the forest soil
was screened for cellulase production. The cellulase activity of YES5 was
optimized via RSM. The saccharification potential of YES5 using Napier
biomass as substrate was evalauted.
Results:
The maximum cellulase activity obtained after optimizing pH, temperature, and
incubation period was 35.70 U. A reliable statistical model was developed for
maximizing the cellulase activity in YES5 Trichosporon asahii. The
cellulase activity was 23.87U, when carbon source in CMC medium was replaced
by Napier biomass. The maximum saccharification potential of 33.15% was
observed on 3rd day.
Interpretation: The study of optimizing the media
composition of Trichosporon asahii cellulase using Napier biomass, a
natural source of carbon for maximizing the cellulase production via RSM, is
first of its kind.
Key words: Cellulase, Napier biomass, Saccharification, Trichosporon
asahii
|