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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Jul 2021, 42 (4)                                     Back


nstantaneous and historical temperature effects on a-pinene

Influence of enzymes and extraction conditions on high yield of cottonseed milk

 

S. Thirukkumar1*, G. Hemalatha1, S. Vellaikumar2, M. Murugan3 and S. Amutha1 

1Department of Food Science and Nutrition, Community Science College and Research Institute, Tamil Nadu Agricultural University, Madurai- 625 104, India

2Department of Biotechnology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai-625 104, India

3Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore- 641 003, India

*Corresponding Author Email : psthirukumar@gmail.com

 

 

Received: 24.12.2020                                                                                  Revised: 25.02.2021                                          Accepted: 03.03.2021

 

 

 

Abstract

Aim: This research aimed to optimize suitable hydrolytic enzymes for maximizing cottonseed milk extracts for high cottonseed milk yield, protein content and low gossypol level.

Methodology: Known amount of cottonseed was soaked for 90 min at 32°C and blended (cottonseed:water@1:6). Different aliquots of the blended cottonseed slurry were treated with 1% of enzymes viz., protease, cellulase and α-amylase enzyme at pH 7.0 followed by incubation at 40 and 52°C for 2.30 hr for the extraction of cottonseed milk. The enzyme activity of extracted milk was subsequently inactivated by pasteurization (90°C, 5 min). Further analysis of physico-chemical characteristics was also carried. The control sample included milk extraction from non-enzyme treated cottonseed milk extract (30±2°C).

Results: Among different treatments, cottonseed milk extraction using protease enzyme at 40°C incubation showed the highest milk yield (86.71%) with the lowest sedimentation (3.72%). Further incubation 40°C and 52°C showed the highest protein content of 2.10 and 2.27 g 100 ml-1 and gossypol reduction of 40.36 and 35.22%, respectively, in the cottonseed milk extract. Meanwhile, cellulase and α-amylase enzymes treated samples at both incubation temperatures showed poor physico-chemical characteristics as compared to control.      

Interpretation: Protease enzyme seems to be the most suitable for optimum or higher extraction of cottonseed milk.       

Key words: Cottonseed milk, Enzymes, Gossypol, Protein

 

 

 

 

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