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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Jul 2021, 42 (4)                                     Back


nstantaneous and historical temperature effects on a-pinene

Antibiotic susceptibility of endosymbionts of papaya mealybug Paracoccus marginatus Williams and Granara de Willink (Hemiptera: Pseudococcidae)

 

P. Megaladevi and J. S. Kennedy* 

Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore– 641 005, India

*Corresponding Author Email : jskennedy@tnau.ac.in

 

 

Received: 24.12.2020                                                                              Revised: 25.02.2021                                                       Accepted: 03.03.2021

 

 

 

Abstract

Aim: The present study aimed to select a powerful tool to evaluate the antibiotic susceptibility of endosymbionts of papaya mealybug.

Methodology: Antibiotic Ofloxacin (0.25µg) was used to disrupt the endosymbionts of mealybug. The antibiotic susceptibility of endosymbionts was studied by conventional method; isolation of symbionts in Luria Bertani Agar and Nutrient Agar and also metagenomic analysis was conducted using nanopore sequencing of 16S rRNA gene.

Results: Molecular identification of isolates showed Gram-positive bacteria i.e., Bacillus cluasii, B. altitudinis, B. siamensis and Gram-negative bacteria, i.e., Serratia marcescens and Stenotrophomonas maltophilia. Metagenomic analysis using 16S amplicon sequencing resulted in identification of  Candidatus Tremblaya Princeps (55%) was more abundant followed by Candidatus Mikella endobia (23%) in untreated mealybug population. Their population was reduced to 7% in Candidatus Tremblaya Princeps and below 1% in Candidatus Mikella endobia.      

Interpretation: The current study confirms that the metagenomic analysis acts as a power lens to evaluate the antibiotic elimination of endosymbionts, hence with this method future works on developing aposymbiotic mealybugs can be done.       

Key words: Endosymbiont, Metagenomic analysis, Ofloxacin, Papaya mealybug

 

 

 

 

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